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Anti-β2-Adrenergic Receptor (extracellular) Antibody

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

Cat #: AAR-016
Alternative Name Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2 (Accession P18762). Extracellular, N-terminus.
Accession (Uniprot) Number P18762
Gene ID 11555
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical.
RRID AB_2039718.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.7 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat heart (lanes 1 and 3) and lung (lanes 2 and 4) lysates:
    Western blot analysis of rat heart (lanes 1 and 3) and lung (lanes 2 and 4) lysates:
    1,3. Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016), (1:200).
    2,4. Anti-β2-Adrenergic Receptor (extracellular) Antibody, preincubated with β2-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR016).
Immunohistochemistry
  • Expression of β2-Adrenoceptor in rat lung
    Expression of β2-Adrenoceptor in rat lung
    Immunohistochemical staining of paraffin-embedded section of rat lung using Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016), (1:100). β2-Adrenoceptor is expressed in the respiratory epithelium. Hematoxilin is used as the counterstain.
Indirect flow cytometry
  • Cell surface detection of β2-Adrenergic Receptor by indirect flow cytometry in live intact mouse J774 macrophage cells:
    Cell surface detection of β2-Adrenergic Receptor by indirect flow cytometry in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC
    ___ Cells + Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016), (2.5μg) + goat-anti-rabbit-FITC
References
  1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS
  2. Piascik, M.T. and Perez, D.M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
  3. Pullar, C.E. et al. (2006) FASEB J. 20, 76.
  4. Oostendorp, J. et al (2005) Am. J. Respir. Crit. Care Med. 172, 322.
  5. Giembycz, M.A. and Newton, R. (2006) Eur. Respir. J. 27, 1286.
Scientific background

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.
 
Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2
 
β2-Adrenoceptors are mainly postsynaptic receptors. They are expressed on a number of tissues including blood vessels, bronchi, gastrointestinal tract (GIT), skeletal muscle, liver and mast cell.3 Functional β2-adrenoceptors were also found on lymphocytes, macrophages and neutrophils.4,5 They are also the only β-adrenoceptor subtype expressed in keratinocytes, fibroblasts and melanocytes.3 Activation of β2-adrenoceptors results in vasodilation, relaxation of the GIT, inhibition of histamine release from mast cells and bronchodilation. Selective agonists of β2-adrenoceptors are used to treat asthma and other related bronchospastic conditions such as Chronic obstructive pulmonary disease (COPD).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2021

Anti-β2-Adrenergic Receptor (extracellular) Antibody (#AAR-016) is a highly specific antibody directed against an extracellular epitope of the mouse β2-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize β2AR from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Western blot citations
  1. Human and rat detrusor lysate.
    Sliva, I. et al. (2020) Br. J. Pharmacol. 177, 1589.
  2. Rat DRG lysate (1:1000).
    Zhu, L. et al. (2015) Sci. Rep. 5, 16109.
Immunohistochemistry citations
  1. Human and rat detrusor sections.
    Sliva, I. et al. (2020) Br. J. Pharmacol. 177, 1589.
  2. Rat brain sections.
    Igata, S. et al. (2014) J. Neurophysiol. 111, 777.
  3. Rat gastric corpus sections (1:200).
    Song, J. et al. (2014) Transl. Res. 164, 22.
Immunofluorescence citations
  1. Rat brain sections.
    Igata, S. et al. (2014) J. Neurophysiol. 111, 777.
  2. Rat gastric corpus sections (1:200).
    Song, J. et al. (2014) Transl. Res. 164, 22.

Specifications

Scientific Background

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