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Anti-α2B-Adrenergic Receptor (extracellular) Antibody

Alpha-2B adrenoceptor, Alpha-2B adrenoreceptor, Alpha-2BAR, ADA2B, ADRA2B

Cat #: AAR-021
Alternative Name Alpha-2B adrenoceptor, Alpha-2B adrenoreceptor, Alpha-2BAR, ADA2B, ADRA2B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
May also work in: h*, m*
Immunogen
  • Peptide (C)RPEPRGLPQ*SELNQE, corresponding to amino acid residues 160-174 of rat α2B-adrenoceptor with replacement of cysteine 169 (C169) with serine (*S) (Accession P19328). 2nd extracellular loop.
Accession (Uniprot) Number P19328
Gene ID 24174
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical; human - 10/14 amino acid residues identical.
RRID AB_10556617.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 5), lung (lanes 2 and 6), liver (lanes 3 and 7) lysates and rat skeletal muscle membranes (lanes 4 and 8):
    Western blot analysis of rat kidney (lanes 1 and 5), lung (lanes 2 and 6), liver (lanes 3 and 7) lysates and rat skeletal muscle membranes (lanes 4 and 8):
    1-4.  Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), (1:200).
    5-8. Anti-α2B-Adrenergic Receptor (extracellular) Antibody, preincubated with α2B-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR021).
Immunohistochemistry
  • Expression of α2B-Adrenoreceptor in rat lung
    Expression of α2B-Adrenoreceptor in rat lung
    Immunohistochemical staining of rat lung paraffin embedded sections using Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), (1:100). α2B-Adrenoreceptor is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.
References
  1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS
  2. Piascik, M.T. and Perez, D.M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
  3. Link, R.E. et al. (1996) Science 273, 803.
  4. Makaritsis, K.P. et al. (1999) Hypertension 33, 14.
  5. Eason, M.G. and Liggett, S.B. (1992) J. Biol. Chem. 267, 25473.
  6. Pitcher, J.A. et al. (1998) Annu. Rev. Biochem. 67, 653.
  7. Kurose, H. and Lefkowitz, R.J. (1994) J. Biol. Chem. 269, 10093.
Scientific background

Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.

Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2.
 
The α2B-adrenoceptor has a distinct pattern of expression within the brain, liver lung and kidney, and recent studies using the knock out mouse system have shown that disruption of this receptor indeed affects mouse viability3, blood pressure responses to α2-adrenoceptor agonists3 and the hypertensive response to salt loading4.
 
Like the α2A-adrenoceptor subtype, the α2B-adrenoceptor undergoes short term agonist promoted desensitization5. This desensitization is due to the phosphorylation of the receptor by G-protein coupled receptor kinases (GRKs)6 which ultimately promotes uncoupling of the receptor from the G-protein subunit7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat α2B-adrenoceptor. Anti-α2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021) can be used in western blot and immunohistochemistry applications. It has been designed to recognize α2B-adrenoceptor from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Mouse sample:
    Hamlett, E.D. et al. (2020) Neurobiol. Dis. 134, 104616.

Specifications

Scientific Background

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