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Anti-5HT1A Receptor (HTR1A) Antibody

5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A
Cat #: ASR-021
Alternative Name 5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide KKSLNGQPGSGDWRRC, corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine receptor 1A (Accession P19327). 3rd intracellular loop.
Accession (Uniprot) Number P19327
Gene ID 24473
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical.
RRID AB_2040194.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Will not recognize human 5-hydroxytryptamine receptor 1A.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    1,2. Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021), (1:200).
    3,4. Anti-5HT1A Receptor (HTR1A) Antibody, preincubated with 5HT1A Receptor/HTR1A Blocking Peptide (#BLP-SR021).
Immunohistochemistry
  • Expression of 5-Hydroxytryptamine receptor 1A in rat cerebellum
    Expression of 5-Hydroxytryptamine receptor 1A in rat cerebellum
    Immunohistochemical staining of rat cerebellum frozen section using Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021), (1:100). 5-HT1A is expressed in Purkinje cells (red). Hoechst 33342 is used as the counterstain.
References
  1. Fink, K.B. and Gothert, M. (2007) Pharmacol. Rev. 59, 360.
  2. http://www.guidetopharmacology.org/GRAC/ReceptorFamiliesForward?type=GPCR
  3. Hoyer, D. et al. (1994) Pharmacol. Rev. 46, 157.
  4. Dabire, H. (1991) Therapie 46, 421.
Scientific background

Serotonin (5-hydroxytryptamine, 5-HT1) is an important neurotransmitter in the central nervous system (CNS) that exerts its neuronal activity via 5-HT receptors.

5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1  The 5-HT receptor families belong to the G-protein coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels.2

The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E and 5-HT1F) which share 41-63% overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation.2

The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the CNS, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species.2,3 

Activation of 5-HT1A receptor decreases blood pressure and heart rate, and is involved in learning, memory, relieving anxiety and depression.1,4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat 5-Hydroxytryptamine receptor 1A. Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021) can be used for western blot and immunohistochemistry applications. It has been designed to recognize 5HT1A receptor from rat and mouse samples.

For research purposes only, not for human use
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