Anti-5HT1A Receptor (HTR1A) Antibody

5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A
    Cat #: ASR-021
    Alternative Name 5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide KKSLNGQPGSGDWRRC, corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine receptor 1A (Accession P19327). 3rd intracellular loop.
    • Anti-5HT1A Receptor (HTR1A) Antibody
    Accession (Uniprot) Number P19327
    Gene ID 24473
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical.
    RRID AB_2040194.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Will not recognize human 5-hydroxytryptamine receptor 1A.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-5HT1A Receptor (HTR1A) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
      1,2. Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021), (1:200).
      3,4. Anti-5HT1A Receptor (HTR1A) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-5HT1A Receptor (HTR1A) Antibody
      Expression of 5-Hydroxytryptamine receptor 1A in rat cerebellum
      Immunohistochemical staining of rat cerebellum frozen section using Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021), (1:100). 5-HT1A is expressed in Purkinje cells (red). Hoechst 33342 is used as the counterstain.
    References
    1. Fink, K.B. and Gothert, M. (2007) Pharmacol. Rev. 59, 360.
    2. http://www.guidetopharmacology.org/GRAC/ReceptorFamiliesForward?type=GPCR
    3. Hoyer, D. et al. (1994) Pharmacol. Rev. 46, 157.
    4. Dabire, H. (1991) Therapie 46, 421.
    Scientific background

    Serotonin (5-hydroxytryptamine, 5-HT1) is an important neurotransmitter in the central nervous system (CNS) that exerts its neuronal activity via 5-HT receptors.

    5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1  The 5-HT receptor families belong to the G-protein coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels.2

    The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E and 5-HT1F) which share 41-63% overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation.2

    The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the CNS, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species.2,3 

    Activation of 5-HT1A receptor decreases blood pressure and heart rate, and is involved in learning, memory, relieving anxiety and depression.1,4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat 5-Hydroxytryptamine receptor 1A. Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021) can be used for western blot and immunohistochemistry applications. It has been designed to recognize 5HT1A receptor from rat and mouse samples.

    For research purposes only, not for human use
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