Anti-5HT1A Receptor (HTR1A) Antibody

5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A
    Cat #: ASR-021
    Alternative Name 5-Hydroxytryptamine receptor 1A, Serotonin receptor 1A
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    • Peptide KKSLNGQPGSGDWRRC, corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine receptor 1A (Accession P19327). 3rd intracellular loop.
    • Anti-5HT1A Receptor (HTR1A) Antibody
    Accession (Uniprot) Number P19327
    Gene ID 24473
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical.
    RRID AB_2040194.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Will not recognize human 5-hydroxytryptamine receptor 1A.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-5HT1A Receptor (HTR1A) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
      1,2. Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021), (1:200).
      3,4. Anti-5HT1A Receptor (HTR1A) Antibody, preincubated with the control peptide antigen.
    • Anti-5HT1A Receptor (HTR1A) Antibody
      Expression of 5-Hydroxytryptamine receptor 1A in rat cerebellum
      Immunohistochemical staining of rat cerebellum frozen section using Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021), (1:100). 5-HT1A is expressed in Purkinje cells (red). Hoechst 33342 is used as the counterstain.
    1. Fink, K.B. and Gothert, M. (2007) Pharmacol. Rev. 59, 360.
    3. Hoyer, D. et al. (1994) Pharmacol. Rev. 46, 157.
    4. Dabire, H. (1991) Therapie 46, 421.
    Scientific background

    Serotonin (5-hydroxytryptamine, 5-HT1) is an important neurotransmitter in the central nervous system (CNS) that exerts its neuronal activity via 5-HT receptors.

    5-HT receptors are subdivided into seven families termed 5-HT1 through 5-HT7.1  The 5-HT receptor families belong to the G-protein coupled receptor superfamily with the exception of 5-HT3 receptor class which belongs to the ligand-gated ion channels.2

    The 5-HT1 receptor class comprises of five different receptors (5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E and 5-HT1F) which share 41-63% overall sequence identity and couple preferentially to Gi/Go to inhibit cAMP formation.2

    The 5-HT1A receptor is the most widespread of all the 5-HT receptors. In the CNS, 5-HT1A receptors exist in the cerebral cortex, hippocampus, septum, amygdala and raphe nucelus in high densities, and was also demonstrated in a variety of tissues from various species.2,3 

    Activation of 5-HT1A receptor decreases blood pressure and heart rate, and is involved in learning, memory, relieving anxiety and depression.1,4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat 5-Hydroxytryptamine receptor 1A. Anti-5HT1A Receptor (HTR1A) Antibody (#ASR-021) can be used for western blot and immunohistochemistry applications. It has been designed to recognize 5HT1A receptor from rat and mouse samples.

    For research purposes only, not for human use
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