Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody

5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A
    Cat #: ASR-033
    Alternative Name 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A (Accession P14842). Extracellular, N-terminus.
    • Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
    Accession (Uniprot) Number P14842
    Gene ID 29595
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 9/13 amino acid residues identical.
    RRID AB_2341074.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.9 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
      Western blot analysis of rat DRG (lanes 1 and 4), mouse brain (lanes 2 and 5) and human brain neuroblastoma SH-SY5Y (lanes 3 and 6) lysates:
      1-3. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:200).
      4-6. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Mouse trigeminal ganglion sections (1:500) (Chang, W. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E5491.).
    References
    1. Hutcheson, J.D. et al. (2011) Pharmacol. Ther. 132, 146.
    2. Fitzgerald, L.W. et al. (2000) Mol. Pharmacol. 57, 75.
    3. Lorke, D.E. et al. (2006) BMC Neurosci. 7, 36.
    4. Raote, I. et al. (2007) Chattopadhyay A, editor. Serotonin receptors in neurobiology. Boca Raton, FL: CRC Press. 105.
    Scientific background

    5-HT is involved in a diverse array of physiological and biological processes. In the brain, 5-HT affects sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. They are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.

    5-HT2A receptors have been found in many regions of the human brain including the cortex, brainstem, olfactory bulb, limbic system and basal ganglia3.

    The 5-HT2A receptor has been implicated in many mental disorders including schizophrenia, depression, obsessive compulsive disorder (OCD), attention deficit–hyperactivity disorder (ADHD), and eating disorders such as anorexia nervosa and autism spectrum disorders4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
    Expression of metabotropic and ionotropic 5-HT Receptors in mouse trigeminal ganglion.
    Immunohistochemical staining of mouse trigeminal ganglion (TG) sections using Alomone Labs Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035) and Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031) (red) shows that all three receptors are expressed in retrogradely labeled whisker afferent neurons.Adapted from Chang, W. et al. (2016) with permission of the National Academy of Sciences, USA.
    Last update: 20/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat 5-HT-2A receptor. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is ideal for detecting the receptor in living cells. It has been designed to recognize rat 5-hydroxytryptamine receptor 2A from rat, mouse, and human samples.

    For research purposes only, not for human use
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