Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody

5-Hydroxytryptamine receptor 5B, Serotonin receptor 5B, MR22
    Cat #: ASR-040
    Alternative Name 5-Hydroxytryptamine receptor 5B, Serotonin receptor 5B, MR22
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide CSDSPSSGRSMG, corresponding to amino acid residues 22-33 of rat 5-Hydroxytryptamine receptor 5B (Accession P35365). Extracellular, N-terminus.
    • Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody
    Accession (Uniprot) Number P35365
    Gene ID 79247
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical.
    RRID AB_2756797.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*, lci*
    Western blot
    • Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody
      Western blot analysis of rat and mouse brain lysates:
      1,2. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody (#ASR-040), (1:400).
      3,4. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody, preincubated with the negative control antigen.
    References
    1. Pithadia, A.B. and Jain, S.M. (2009) J. Clin. Med. Res. 1, 72.
    2. Venkatakrishnan A.J. et al. (2013) Nature 494, 185.
    3. Hannon, J. and Hoyer, D. (2008) Behav. Brain Res. 195, 198.
    Scientific background

    Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid tryptophan by the enzyme tryptophan-decarboxylase. This conversion occurs in chromaffin and enteric cells whereas serotonin reaches the central nervous system via blood circulation. In addition to its function as a neurotransmitter in the CNS, it serves as a precursor for melatonin in the pineal gland. It can also be found in non-neuronal tissues such as the mucosa of gut and blood platelets. Serotonin is known to affect numerous behavioral and physiological functions1.

    The 5-HT5B receptor is part of family of 13 known G-protein coupled receptors (GPCRs) divided into seven classes based on structural and operational features. Like other GPCRs, 5-HT5B receptor has an extracellular N-terminus, transmembrane domains and an intracellular C-terminal tail2.

    There are currently two known receptors in the 5-HT5 family. The function of these receptors is far from fully understood and it is unclear with which G-protein they couple. The 5-HT5B gene has been mapped in the human genome but its sequence does not produce a functional gene and no physiological response was found to correspond with its activity3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat 5-Hydroxytryptamine receptor 5B. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody (#ASR-040) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize 5HT5B receptor from rat and mouse samples.

    For research purposes only, not for human use
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