Name: Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ASR-037-F

Overview

Cat #: ASR-037-F

Alternative Name 5-Hydroxytryptamine Receptor 7, Serotonin Receptor 7, 5-HT7, 5-HT-7

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CGEQINYGRVEK, corresponding to amino acid residues 73 - 84 of rat 5-HT7 (Accession P32305). Extracellular, N-terminus.

Accession (Uniprot) Number P32305

Gene ID 65032

Homology Mouse, human - identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fuorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASR-037), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of 5HT7 Receptor by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody (#ASR-037-F), (2.5µg).
Cell surface detection of 5HT7 Receptor by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody (#ASR-037-F), (5µg).

References

Ruat, M. et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 8547.
Hedlund, P.B. and Sutcliffe, J.G. (2004) Trends Pharmacol. Sci. 25, 481.
Vanhoenacker, P. et al. (2000) Trends Pharmacol. Sci. 21, 70.
Thirumaran, S.L. et al. (2019) Eur. J. Med. Chem. 183, 111705.

Scientific background

5-HT7 receptor is a G-protein coupled receptor (GPCR) that is activated by Serotonin. Like all other members, 5-HT7 has seven transmembrane domains. In addition, it contains a hydrophobic domain located at its N-terminal end¹.

The signaling cascades activated by these receptors take part in circadian rhythm, learning and memory, hippocampal signaling and smooth muscle relaxation. It was also shown to have a role in several disorders including Autism, neuropsychiatric disorders, Epilepsy and Alzheimer's disease^2,4.

5-HT7 is encoded by HTR7 gene and it has three splice variants encoding proteins that differ in the length of their carboxy-terminal ends. The splice variants are differentially expressed in the spleen, kidney, heart, thalamus, hindbrain, hippocampus, cortex, caudate, striatum and cerebellum³.

Due to its involvement in central and the peripheral nervous systems, endocrine system and its involvement in various diseases and syndromes, 5-HT7 receptor represents an interesting target for the treatment and prevention of various pathologies. Selective ligands are being studied in order to regulate this receptor's activity⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize 5HT7 receptor from rat, mouse and human samples.

Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody (#ASR-037-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize 5HT7 receptor from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 02/01/2024

Applications

Citations

Specifications

Scientific Background

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Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody