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Anti-ABCB4/MDR3 Antibody

ATP binding cassette subfamily B member 4, Multidrug resistance protein 3, P-glycoprotein 2, Pgy2

Cat #: AMT-023
Alternative Name ATP binding cassette subfamily B member 4, Multidrug resistance protein 3, P-glycoprotein 2, Pgy2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
  • Peptide (C)KARIFRNSTKKSLK, corresponding to amino acid residues 658-671 of rat ABCB4/MDR3 (Accession Q08201). 3rd intracellular loop.
Accession (Uniprot) Number Q08201
Gene ID 24891
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4):
    Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4):
    1,2. Anti-ABCB4/MDR3 Antibody (#AMT-023), (1:200).
    3,4. Anti-ABCB4/MDR3 Antibody, preincubated with ABCB4/MDR3 Blocking Peptide (#BLP-MT023).
  1. Schatz, S.B. et al. (2018) Hepatol. Commun. 2, 504.
  2. Hu, H. et al. (2018) Biosci. Rep. 38, 1.
  3. Venot, Q. et al. (2016) PLoS One 11, e0146962.
Scientific background

ABCB4 (MDR3) is a member of the ABC transporter superfamily. ABC transporters comprise a large number of membrane proteins, which mediate the translocation of a variety of molecules across cellular membranes. MDR3 is a transporter expressed at the canalicular membrane of hepatocytes, where it translocates phosphatidylcholine (PC) in bile in order to protect the hepatobiliary epithelial against the damage from free bile acids1-3.

Genetic variants in the ABCB4 gene sequence are associated with rare biliary diseases, in particular progressive familial intrahepatic cholestasis type 3. Different phenotypes are detected in ABCB4 mutations such as, low phospholipid‐associated cholelithiasis and intrahepatic cholestasis of pregnancy1-3.

ABCB4 is a polytopic transmembrane glycoprotein with two transmembrane modules each spanning the membrane six times and two ATP-binding domains. The protein possesses a conserved C-terminal region. The presence of a hydrophobic amino acid at the C-terminus suggests properties of a PDZ-binding-like motif2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-ABCB4/MDR3 Antibody (#AMT-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize MDR3 from rat and mouse samples. It is unlikely to recognize the protein from human samples.

For research purposes only, not for human use



Scientific Background

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