Anti-Alpha 1 Na+/K+ ATPase Antibody

ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1
    Cat #: ANP-001
    Alternative Name ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7- 20 of rat ATP1A1 (Accession P06685). Intracellular, N-terminus.
        Anti-Alpha 1 Na+/K+ ATPase Antibody
    Accession (Uniprot) Number P06685
    Gene ID 24211
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, dog, pig – identical; Human- 13/14 amino acid residues identical.
    RRID AB_2341003.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
      • Anti-Alpha 1 Na+/K+ ATPase Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
        1,2. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).
        3,4. Anti-Alpha 1 Na+/K+ ATPase Antibody, preincubated with the negative control antigen.
        Anti-Alpha 1 Na+/K+ ATPase Antibody
        Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:
        1. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).
        2. Anti-Alpha 1 Na+/K+ ATPase Antibody, preincubated with the negative control antigen.
        Note: Samples should be heated up to 37°C. Do not boil samples.
      • Anti-Alpha 1 Na+/K+ ATPase Antibody
        Expression of Na+/K+ ATPase α1 (ATP1A1) in rat kidney
        Immunohistochemical staining of paraffin embedded rat kidney sections using Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200). ATP1A1 staining (brown) is present in collecting ducts and thick portions of the Henle loop in the renal medulla. Hematoxilin is used as the counterstain.
        Anti-Alpha 1 Na+/K+ ATPase Antibody
        Expression of Na+/K+ ATPase α1 (ATP1A1) in mouse brain
        Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:400). ATP1A1 staining (red) is apparent in the hippocampal dentate gyrus in neuronal soma (vertical arrows) and in astrocytes (horizontal arrow). DAPI (blue) is used to visualize cell nuclei.
    References
      • Na+/K+-ATPase, a membrane protein located on the plasma membrane of all animal cells, is involved in maintaining the Na+ and K+ gradients across the cell membrane1. It is ubiquitously expressed in neurons and helps maintain normal neuronal function2. The enzyme is composed of a catalytic α chain of 112 kDa. and α glycosylated β chain of 40–50 kDa.3. There are four isoforms of the α subunit (α1, α2, α3 and α4) and three isoforms of the β subunit (β1, β2 and β3) with different tissue distributions4.
         
        The N-terminus is located on the cytoplasmic side of the membrane. There are four transmembrane segments in the first third of the polypeptide chain, a large cytoplasmic loop (LCL) comprising the second third of the chain and four to six transmembrane segments in the C-terminal third of the protein with the C-terminus also on the cytoplasmic side of the membrane5.
         
        Na+/K+-ATPase abnormalities have been reported to be involved in several neurological diseases such as seizures, bipolar disorder, spongiform encephalopathy, and Alzheimer’s disease. Na+/K+-ATPase may also have implications in behavioral defects6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 19/08/2019

    Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001) is a highly specific antibody directed against an epitope of the rat ATP1A1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A1 from rat, mouse, and human samples.

    For research purposes only, not for human use