Anti-GluR3 (GluA3) (extracellular) Antibody

AMPA Receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3
Cat #: AGC-010
Alternative Name AMPA Receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat GluR3 (Accession P19492). Extracellular, N-terminus.
    Accession (Uniprot) Number P19492
    Gene ID 29628
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human, dog - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, lci, wb
    May also work in: ifc
      • Western blot analysis of rat cerebellum lysates:
        1. Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010), (1:400).
        2. Anti-GluR3 (GluA3) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Immunoprecipitation of rat cerebellum lysates:
        1. Cerebellum lysates.
        2. Cerebellum lysates + Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010) + protein A  beads.
        3. Cerebellum lysates + pre-immune rabbit serum + protein A beads.

        Black arrow indicates GluR3 while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010).
      • Expression of GRIA3 (GluR3) in mouse cerebellum
        Immunohistochemical staining of frozen perfusion-fixed free floating sections of mouse cerebellum using Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010). A. Distribution of GRIA3 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that GRIA3 is localized to Bergmann glia (vertical arrow) and to Purkinje cell soma (horizontal arrow). DAPI is used as the counterstain (blue).
      • Rat hippocampal neurons (Parkinson, G.T. et al. (2018) Sci. Rep. 8, 4155.).
      • Rat hippocampal neurons (Koszegi, Z. et al. (2017) Sci. Rep. 7, 12318.).
      • L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors).

        The ligand-gated ion channel family consists of 15 members that have been subdivided into three families based on their pharmacological profile: the a-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) preferring receptors, the N-methyl-D-aspartate (NMDA) preferring and the kainate preferring receptors.

        The AMPA receptor subfamily includes four members AMPA1 to AMPA4, also known as GluR1 to GluR4 respectively.

        The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3 channels, although homotetramers can also been found.
        AMPA receptors are permeable to cations Na+, K+ and Ca2+.  The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+.1

        Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane potential.

        Synaptic strength that is defined as the level of post-synaptic depolarization can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.2

        The exact physiological role of the AMPA3 receptor is not clear but a role in the modulation of oscillatory networks affecting sleep and breathing has been suggested.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 05/06/2019

    Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010) is a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 3. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and immunoprecipitation applications. It has been designed to recognize GluR3 from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
      • Mouse brain lysate. Also tested in GRIA3-/- mice.
        Garcia-Hernandez, S. et al. (2017) Hearing Res. 344, 284.

      • Mouse brain lysate. Also tested in GRIA3-/- mice.
        Garcia-Hernandez, S. et al. (2017) Hearing Res. 344, 284.

      • Rat hippocampal lysate.
        Koszegi, Z. et al. (2017) Sci. Rep. 7, 12318.

      • Rat brain lysate (1:500).
        Russo, I. et al. (2013) Neuromolecular Med. 15, 324.

      • Rat brain lysate.
        Barbon, A. et al. (2011) Neurochem. Int. 59, 896.

      • Rat hippocampal neurons.
        Koszegi, Z. et al. (2017) Sci. Rep. 7, 12318.

      • Rat hippocampal neurons.
        Parkinson, G.T. et al. (2018) Sci. Rep. 8, 4155.

      • Transfected rat organotypic cultures.
        Hamad, M.I. et al. (2011) Development 138, 4301.

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