Anti-Aquaporin 7 Antibody

AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose
    Cat #: AQP-007
    Alternative Name AQP7, Aquaglyceroporin-7, AQPap, Aquaporin adipose
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide corresponding to amino acid residues 7-24 of rat AQP7 (Accession P56403). Intracellular, N-terminus.
        Anti-Aquaporin 7 Antibody
    Accession (Uniprot) Number P56403
    Gene ID 29171
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 15/18 amino acid residues identical; human - 11/18 amino acid residues identical.
    RRID AB_2039740.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-Aquaporin 7 Antibody
        Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) kidney membranes:
        1,2. Anti-Aquaporin 7 Antibody, (#AQP-007), (1:500).
        3,4. Anti-Aquaporin 7 Antibody, preincubated with the control peptide antigen.
      • Anti-Aquaporin 7 Antibody
        Expression of AQP7 in rat kidney
        Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 7 Antibody (#AQP-007), (1:100). A. In the minor calyx, AQP7 staining (deep red) is specific for epithelial cells of the uroepithelium  B. In the cortical labyrinth, AQP7 staining is specific for renal tubules while the stronger staining is evident in the brush border (blue arrows). Hematoxilin is used as the counterstain.
      • Anti-Aquaporin 7 Antibody
        Expression of AQP7 in rat adipocytes
        Immunocytochemical staining of 3T3-L1 adipocytes using Anti-Aquaporin 7 Antibody (#AQP-007), (1:800), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red) A, C. Anti-Aquaporin 7 Antibody preincubated with the control peptide antigen followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody. B, D. Visible light images of the adipocytes shown in (A) and (C) respectively.
    References
    • 1. Kuriyama, H. et al. (1997) Biochem. Biophys. Res. Commun. 241, 53.
    • 2. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
    • 3. Fruhbeck, G. et al. (2006) Trends Pharmacol. Sci. 27, 345.
      • Aquaporin 7 (AQP-7) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.

        The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-7, AQP-3, AQP-9 and AQP-10.

        The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.

        AQP-7 is expressed in ovary, testis, kidney and adipose tissue.

        The function of AQP-7 in adipose tissue attracted much interest as mice deficient in AQP-7 developed adult-onset obesity and Type 2 Diabetes. AQP-7 modulates adipocyte glycerol permeability thereby controlling triglyceride accumulation and fat cell size.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Aquaporin 7 Antibody (#AQP-007) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize AQP-7 from mouse and rat samples, and may recognize the channel from human samples.

    For research purposes only, not for human use