Anti-Aquaporin 8 Antibody

AQP8
    Cat #: AQP-008
    Alternative Name AQP8
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: r
      • Peptide (C)REIKGKETNMADSYH, corresponding to amino acid residues 14-28 of rat AQP8 (Accession P56405). Intracellular, N-terminus.
        Anti-Aquaporin 8 Antibody
    Accession (Uniprot) Number P56405
    Gene ID 29172
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Unlikely to recognize mouse or human samples.
    RRID AB_2039742.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-Aquaporin 8 Antibody
        Western blot analysis of rat small intestine membranes:
        1. Anti-Aquaporin 8 Antibody (#AQP-008), (1:200).
        2. Anti-Aquaporin 8 Antibody, preincubated with the control peptide antigen.
        Anti-Aquaporin 8 Antibody
        Western blot analysis of rat pancreas membranes:
        1. Anti-Aquaporin 8 Antibody (#AQP-008), (1:400).
        2. Anti-Aquaporin 8 Antibody, preincubated with the control peptide antigen.
      • Anti-Aquaporin 8 Antibody
        Expression of AQP8 in rat pancreas
        Immunohistochemical staining of rat pancreas paraffin-embedded sections using Anti-Aquaporin 8 Antibody (#AQP-008), (1:100). Staining (brown) is specific for the apical portion of acinar cells in the exocrine pancreas. Hematoxilin is used as the counterstain.
        Anti-Aquaporin 8 Antibody
        Expression of AQP8 in rat lung
        Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-Aquaporin 8 Antibody (#AQP-008), (1:100). Staining (brown) is specific for the bronchiolar epithelium. Hematoxilin is used as the counterstain.
    References
      • Aquaporin 8 (AQP-8) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.

        The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include besides AQP-8, AQP-1, AQP-2, AQP4 and AQP-5, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.

        The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.2

        AQP-8 is expressed along the gastrointestinal tract including small intestine, colon, pancreas and liver and also in other organs such as testis, lung and kidney.

        The physiological function of AQP-8 is not entirely clear but studies suggest that it has an important role in colonic water adsorption and hepatocyte bile formation.3,4 Interestingly, AQP-8 expression is markedly altered in gastrointestinal disorders such as ulcerative colitis and inflammatory bowel disease.5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Aquaporin 8 Antibody (#AQP-008) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP8 from rat samples only.

    For research purposes only, not for human use
    Citations
      • Rat digestive system lysate (1:400).
        Zhao, G. et al. (2016) Biotech. Histochem. 91, 269.
      • Rat colon and ileum lysates (1:400).
        Zhao, G. et al. (2014) Biochem. Biophys. Res. Commun. 443, 161.
      • Rat digestive system sections (1:100).
        Zhao, G. et al. (2016) Biotech. Histochem. 91, 269.
      • Rat colon and ileum sections (1:100).
        Zhao, G. et al. (2014) Biochem. Biophys. Res. Commun. 443, 161.
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