Anti-Aquaporin 9 Antibody

AQP9, Aquaglyceroporin-9
    Cat #: AQP-009
    Alternative Name AQP9, Aquaglyceroporin-9
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
      • Peptide (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP9 (Accession P56627). Intracellular, N-terminus.
        Anti-Aquaporin 9 Antibody
    Accession (Uniprot) Number P56627
    Gene ID 65054
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 13/16 amino acid residues identical.
    RRID AB_2039744.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Unlikely to recognize human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
      • Anti-Aquaporin 9 Antibody
        Western blot analysis of rat brain (lanes 1 and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes:
        1,2,3. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
        4,5,6. Anti-Aquaporin 9 Antibody, preincubated with the control peptide antigen.
        Anti-Aquaporin 9 Antibody
        Western blot analysis of rat H4-II-E-C3 hepatoma cell line lysate:
        1. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
        2. Anti-Aquaporin 9 Antibody, preincubated with the control peptide antigen.
        Anti-Aquaporin 9 Antibody
        Western blot analysis of mouse brain membranes:
        1. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
        2. Anti-Aquaporin 9 Antibody, preincubated with the control peptide antigen.
      • Anti-Aquaporin 9 Antibody
        Expression of AQP9 in rat liver
        Immunohistochemical staining of rat liver paraffin embedded sections using Anti-Aquaporin 9 Antibody (#AQP-009) (1:100). AQP9 (brown) is expressed in hepatocytes of the liver parenchyma. Hematoxilin is used as the counterstain.
        Anti-Aquaporin 9 Antibody
        Expression of AQP9 in rat epididymis
        Immunohistochemical staining of rat testis paraffin embedded section using Anti-Aquaporin 9 Antibody (#AQP-009), (1:100). AQP9 (brown) is expressed in the columnar epithelium of the epididymus (arrows). Hematoxilin is used as the counterstain.
    References
    • 1. Rojek, A.M. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 3609.
    • 2. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
    • 3. Koyama, Y. et al. (1997) J Biol Chem 272, 30329.
      • Aquaporin 9 (AQP-9) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.

        The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water (AQP-1, AQP-2, AQP4, AQP-5, AQP-6, AQP-8), and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.

        The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.2

        AQP-9 is expressed in several tissues including the brain, liver, testis and spleen. In the liver AQP-9 has a central role in glycerol metabolism as demonstrated in experiments using AQP-9 knockout mice: mice deficient in AQP-9 expression showed enhanced plasma levels of glycerol and triglycerides.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Aquaporin 9 Antibody (#AQP-009) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP9 from mouse and rat samples.

    For research purposes only, not for human use