Anti-ASCT1 Antibody

Alanine/Serine/Cysteine Transporter 1, Alanine/Serine/Cysteine/Threonine Transporter 1, Neutral amino acid transporter A, SLC1A4
    Cat #: ANT-081
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)SKSEEETSPLVTH, corresponding to amino acid residues 500-512 of mouse ASCT1 (Accession O35874). Intracellular, C-terminus.
    Accession (Uniprot) Number O35874
    Gene ID 55963
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – identical; human – 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1, 2. Anti-ASCT1 Antibody (#ANT-081), (1:200).
    3, 4. Anti-ASCT1 Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human Colo 205 colon adenocarcinoma cell line lysate (lanes 2 and 4):
    1, 2. Anti-ASCT1 Antibody (#ANT-081), (1:200).
    3, 4. Anti-ASCT1 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of ASCT1 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ASCT1 Antibody (#ANT-081), (1:300), followed by goat-anti-rabbit-AlexaFluor-488. ASCT1 staining (green) is detected in the sub-granular layer (SGL, arrows) of the dentate gyrus. GL = granule layer. Cell nuclei are stained with DAPI (blue).
    References
    1. Scopelliti, A.J. et al. (2013) J. Biol. Chem. 288, 8250.
    2. Damseh, N. et al. (2015) J. Med. Genet52, 541.
    Scientific background

    Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells throughout the body with glutamate for metabolic purposes.

    SLC1A4 belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters such as the human glutamate transporters (otherwise known as excitatory amino acid transporters or EAATs).

    SLC1A4 is detected in the brain, skeletal muscle, and pancreas. It is largely selective for alanine, serine, and cysteine but can also transport threonine, asparagine, and to a lesser extent, proline. In the brain, SLC1A4 is preferentially expressed in glial cells and has been suggested to be responsible for the release of L-serine from intracellular stores, in exchange for other extracellular substrates. SLC1A4 exhibits pH-dependent substrate selectivity. Acidic amino acids become substrates at pH 5.5, which may be physiologically important in the acidic environment of the digestive tract where SLC1A4 is involved in nutrient uptake.

    Despite the high degree of sequence identity, ASCTs function quite differently from the EAATs. The EAATs transport the acidic amino acids glutamate and aspartate against a concentration gradient generating a net movement of substrate and charge across the membrane. In contrast, SLC1A4 exchanges small, neutral amino acids in an electroneutral fashion without generating net movement of substrate1.

    Recent studies have shown that mutations in the SCL1A4 gene, which are common in Ashkenazi Jews, are associated with global developmental delay, microcephaly and hypomyelination2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Immuno-colocalization of GluN1 and ASCT1 in mouse hippocampus.Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGP-046), (1:600) and rabbit Anti-ASCT1 Antibody (#ANT-081), (1:300). A. GluN1 (red) appears in profiles of hippocampal dentate gyrus interneurons (arrows). B. ASCT1 (green) appears in the extracelullar matrix of the subgranular layer (SGL). C. Merge of the two images demonstrates adjacent localization in several neurons (arrows). Cell nuclei are stained with DAPI (blue).GL = granule layer.

    Last update: 29/10/2018

    Anti-ASCT1 Antibody (#ANT-081) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC1A4 from human, mouse, and rat samples.

    For research purposes only, not for human use