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Anti-ASIC2a Antibody

ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG

Cat #: ASC-012
Alternative Name ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide DLKESPSEGSLQPSSIQC, corresponding to amino acid residues 2-18 of human ASIC2a (Accession Q16515). Intracellular, N-terminus.
    Accession (Uniprot) Number Q16515
    Gene ID 40
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    RRID AB_2039699.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Specificity The antibody is specific for ASIC2a and will not recognize ASIC2b.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Western blot analysis of rat brain membranes:
      Western blot analysis of rat brain membranes:
      1. Anti-ASIC2a Antibody (#ASC-012), (1:200).
      2. Anti-ASIC2a Antibody, preincubated with ASIC2a Blocking Peptide (#BLP-SC012).
    Immunoprecipitation
    • D54-MG transfected cell lysate (Vila Carriles, W.H. et al. (2007) J. Biol. Chem. 282, 34381.).
    Immunohistochemistry
    • Rat DRG sections (Alvarez de la Rosa, D. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 2326.).
    • Expression of ASIC2a in rat spinal cord. 
      Expression of ASIC2a in rat spinal cord. 
      Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-ASIC2a Antibody(#ASC-012), (1:300), followed by donkey anti-rabbit-Cy3. A. ASIC2a immunoreactivity (red) appeared in neuronal soma (horizontal arrow) in the ventral horn of the spinal cord. B. Pre-incubation of the antibody with ASIC2a Blocking Peptide (BLP-SC012), suppressed staining. Cell nuclei are stained with DAPI (blue). 
    Immunocytochemistry
    • Mouse dendritic cells (Tong, J. et al. (2011) J. Immunol. 186, 3686.).
    References
    1. Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735.
    2. Krishtal, O. et al. (2003) Trends Neurosci. 26, 477.
    3. Price, M.P. et al. (2000) Nature 407, 1007.
    Scientific background

    ASIC2a is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC3ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.

    There are two recognized splice variants of the ASIC2 gene that differ on their N-termini, ASIC2a and ASIC2b that have different tissue distributions and functions. ASIC2a is highly expressed in the central nervous system (CNS) and is less expressed in the peripheral nervous system (PNS) while ASIC2b is prominently expressed in the latter.

    The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits. The ASIC2b splice variant does not appear to be functional when expressed alone but it can modify the properties of ASIC2a and ASIC3 when co-expressed.

    The ASIC2 protein has been proposed to be involved in mechanosensation and sensory transduction.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-ASIC2a Antibody
    Expression of ASIC2a in rat medulla.Immunohistochemical staining of rat brain sections using Anti-ASIC2a Antibody (#ASC-012). ASIC2a staining in the medulla is detected in the ventrolateral medulla (VLM), (left panel) and in the dorsal medulla (DM), (middle panel). Antibody specificity was demonstrated by preincubating the antibody with the blocking peptide (right panel).Adapted from Song, N. et al. (2016) Sci. Rep. 6, 38777. with permission of SPRINGER NATURE.

    Last update: 12/08/2021

    Anti-ASIC2a Antibody (#ASC-012) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize ASIC2a from human, rat, and mouse samples. The antibody is specific for ASIC2a and will not recognize the ASIC2b variant.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Western blot analysis of human primary astrocyte lysate. Tested in siRNA-treated cells.
      Vila-Carriles, W.H. et al. (2007) J. Biol. Chem. 282, 34381.
    Western blot citations
    1. Rat DRG lysate (1:200).
      Wu, Y. et al. (2017) Cell. Mol. Neurobiol. 37, 635.
    2. Mouse bone marrow-derived macrophage (BMM) lysate.
      Kong, X. et al. (2013) Cell. Immunol. 281, 44.
    3. Human SH-SY5Y cell lysate.
      Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.
    4. Mouse immature dendritic cells.
      Tong, J. et al. (2011) J. Immunol. 186, 3686.
    5. Mouse cortex lysate (1:200).
      Hu, Z.L. et al. (2010) Am. J. Physiol. 299, C1355.
    6. Mouse brain lysate.
      Joch, M. et al. (2007) Mol. Biol. Cell 18, 3105.
    7. Human primary astrocyte lysate. Also tested in siRNA-treated cells.
      Vila-Carriles, W.H. et al. (2007) J. Biol. Chem. 282, 34381.
    Immunohistochemistry citations
    1. Rat DRG sections (1:50).
      Wu, Y. et al. (2017) Cell. Mol. Neurobiol. 37, 635.
    2. Mouse hippocampus sections (1:200).
      Ferenczi, E.A. et al. (2016) Sci. Rep. 6, 23947.
    3. Rat medulla tissue sections.
      Song, N. et al. (2016) Sci. Rep. 6, 38777.
    4. Rat DRG sections.
      Alvarez de la Rosa, D. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 2326.
    Immunocytochemistry citations
    1. Mouse bone marrow-derived macrophages (BMMs).
      Kong, X. et al. (2013) Cell. Immunol. 281, 44.
    2. Human SH-SY5Y cell lysate.
      Xiong, Q.J. et al. (2012) Am. J. Physiol. 303, C376.
    3. Mouse dendritic cells.
      Tong, J. et al. (2011) J. Immunol. 186, 3686.
    More product citations
    1. Zhang, Y. et al. (2012) Neurol. Sci. 33, 1125.

    Specifications

    Scientific Background

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