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Anti-B1 Bradykinin Receptor (BDKRB1) Antibody

B1R, BKR1, B1BKR, BKRB1, Kinin B1, BRADYB1

Cat #: ABR-011
Alternative Name B1R, BKR1, B1BKR, BKRB1, Kinin B1, BRADYB1
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide (C)KEAS RTR*SG GPKGS K, corresponding to amino acid residues 243-257 of rat B1R with replacement of cysteine 250 (C250) with serine (*S) (Accession P97583). 3rd intracellular loop.
    Accession (Uniprot) Number P97583
    Gene ID 81509
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 13/15 amino acid residues identical.
    RRID AB_2039752.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Might not recognize the human epitope.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Western blot analysis of rat brain (lanes 1 and 3) and heart (lanes 2 and 4) lysates:
      Western blot analysis of rat brain (lanes 1 and 3) and heart (lanes 2 and 4) lysates:
      1-2. Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011), (1:200).
      3-4. Anti-B1 Bradykinin Receptor (BDKRB1) Antibody, preincubated with B1 Bradykinin Receptor/BDKRB1 Blocking Peptide (#BLP-BR011).
    Immunohistochemistry
    • Expression of B1 bradykinin receptor in rat CNS
      Expression of B1 bradykinin receptor in rat CNS
      Immunohistochemical staining of frozen rat brain section (A & B) and cerebellum (C) using Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011), (1:50). BKR1 is expressed in neuronal cells of the amygdala (A) and hippocampus (CA1) (B) as well as in the axons of Purkinje cells in the cerebellum (arrowheads on C). Hoechst 33342 is used as the counterstain.
    • Mouse spinal cord (1:200), (Dutra, R.C. et al. (2013) Neurobiol. Dis. 54, 82.).
    Immunocytochemistry
    • Rat trigeminal neuron primary cultures (Kawaguchi, A. et al. (2015) Front. Cell. Neurosci. 9, 229.).
    References
    1. Walker, K. et al. (1995) Neurochem. Int. 26, 1.
    2. Böckmann, S. and Paegelow, I. (2000) J. Leukoc. Biol. 68, 587.
    3. Hess, J.F. et al. (2004) J. Pharmacol. Exp. Ther. 310, 488.
    Scientific background

    Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function.1

    Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). The desArg9-BK and Lys-desArg9-BK peptides activate BKRB1 while BK and Lys-BK operate by activating BKRB2.2 Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

    Expression of BKRB1is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. BKRB1 was long considered not to be expressed in healthy tissues. However, recent work has demonstrated a low level of expression of BKRB1 in the CNS of rodent and primates. In contrast, BKRB2 is constitutively expressed on various cell types.3 BKRB1 represents a potential therapeutic target for treatment of inflammatory disorders and cardiovascular diseases.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-B1 Bradykinin Receptor (BDKRB1) Antibody
    Expression of B1 bradykinin receptor in rat TG neurons.Immunocytochemical staining of rat primary trigeminal ganglion (TG) neurons using Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011). Adapted from Kawaguchi, A. et al. (2015) Front. Cell. Neurosci. 9, 229. with permission of Frontiers.

    Last update: 31/08/2021

    Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize B1R from rat and mouse samples. The antibody may not recognize the receptor from human samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Immunocytochemistry of primary mouse hypothalamic neurons. Tested in BDKRB1 knockout mice. 
      ParekhR.Uet al. (2021Int. J. Mol. Sci. 22, 145. 
    Immunohistochemistry citations
    1. Rat brain sections (1:200).
      Bitencourt, R.M. et al. (2017) Behav. Brain Res. 316, 74.
    2. Rat trigeminal neuron sections.
      Kawaguchi, A. et al. (2015) Front. Cell. Neurosci. 9, 229.
    3. Rat lung sections.
      Duehrkop, C. et al. (2013) PLoS ONE 8, e72059.
    4. Mouse spinal cord. Also tested in B1R-/- mice (1:200).
      Dutra, R.C. et al. (2013) Neurobiol. Dis. 54, 82.
    Immunocytochemistry citations
    1. Rat trigeminal primary culture.
      Kawaguchi, A. et al. (2015) Front. Cell. Neurosci. 9, 229.

    Specifications

    Scientific Background

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