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Anti-BAI1 (extracellular) Antibody

Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF

Cat #: ABR-021
Alternative Name Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GRVRTYQFDSFLESTR, corresponding to amino acid residues 97-112 of mouse BAI1 (Accession Q3UHD1). Extracellular, N-terminus.
Accession (Uniprot) Number Q3UHD1
Gene ID 107831
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human - identical
RRID AB_2340891.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody will recognize the intact BAI1 receptor as well as the proteolytically processed N-terminal fragment. This fragment is also known as Vasculostatin (Vstat120).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-BAI1 (extracellular) Antibody (#ABR-021), (1:200).
    3,4. Anti-BAI1 (extracellular) Antibody, preincubated with BAI1 (extracellular) Blocking Peptide (#BLP-BR021).
  • Western blot analysis of human HL-60 promyelocytic leukemia cell lysates:
    Western blot analysis of human HL-60 promyelocytic leukemia cell lysates:
    1. Anti-BAI1 (extracellular) Antibody (#ABR-021), (1:200).
    2. Anti-BAI1 (extracellular) Antibody, preincubated with BAI1 (extracellular) Blocking Peptide (#BLP-BR021).
Immunohistochemistry
  • Expression of BAI1 in mouse olfactory bulb
    Expression of BAI1 in mouse olfactory bulb
    Immunohistochemical staining of mouse perfusion-fixed olfactory bulb frozen sections using Anti-BAI1 (extracellular) Antibody (#ABR-021), (1:200). A. BAI1 (green) is expressed in astrocyte-like cells (arrows). B. Double-staining of BAI1 (green) and glial fibrillary acidic protein (red) reveals expression of BAI1 in a subset of astrocytes. Nuclear staining of cells using the DNA dye DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of BAI1 in live intact human HL-60 promyelocytic leukemia cell line:
    Cell surface detection of BAI1 in live intact human HL-60 promyelocytic leukemia cell line:
    ___ Unstained cells + goat-anti-rabbit-AlexaFluor-488 secondary antibody.
    ___ Cells + Anti-BAI1 (extracellular) Antibody (#ABR-021), (1:20) + goat-anti-rabbit-AlexaFluor-488 secondary antibody.
  • The blocking peptide is not suitable for this application.
References
  1. Park, D. et al. (2007) Nature 450, 430.
  2. Cork, S.M. et al. (2011) J. Mol. Med. 89, 743.
  3. Oda, K. et al. (1999) Cytogenet. Cell. Genet. 84, 75.
  4. Cork, S.M. et al. (2012) Oncogene 31, 5144.
  5. Shiratsuchi, T. et al. (1997) Cytogenet. Cell. Genet. 79, 103.
  6. Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5, 181.
  7. Fukushima, Y. et al. (1998) Int. J. Oncol. 13, 967.
Scientific background

The three members of the brain angiogenesis inhibitor (BaI1-3) are receptors belonging to the adhesion subfamily of G-protein coupled receptor superfamily. Like all members of GPCRs, all three BaIs have seven transmembrane domains, an intracellular C-terminal tail and extracellular N-terminus. Like other adhesion members, the N-terminus is quite large1,2. Many domains are localized to the N-terminus; various glycosylations sites are present, there is a GPCR proteolysis site, a putative hormone binding domain and thrombospondin type 1 repeats which regulate the anti-angiogenic activity of thrombospondin-12,3. The C-terminal tail interacts with PDZ-domain proteins. Unique to BaI1 is a proline-rich domain required for interacting with Src homology domains and WW domain proteins2,4.

Like most adhesion GPCRs, BaI also undergo proteolysis at the N-terminus at a highly rich cystein domain2. Following autocleavage, the N-terminal fragment remains associated to the receptor. In BaI1, proteolysis yields a partly secreted 120 kDa. fragment (vasculostatin-120) or a 40 kDa. fragment both having antiangiogenic effects2,5

At the mRNA level, all BaIs are expressed in fetal and adult human brain2,6. BaI2 is detected in the human heart and skeletal muscle. BaI3 is expressed in the human heart, testis and small intestine. In mouse, both BaI2 and BaI3 are restricted to the brain2.

These receptors are implicated in various diseases and disorders such as primary glioma, pulmonary adenocarcinomas, gastric and colorectal cancers2,6,7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-BAI1 (extracellular) Antibody (#ABR-021) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize BAI1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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