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Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody

Sodium-Potassium ATPase Subunit Beta 1, ATP1B, ATP1B1

Cat #: ANP-014
Alternative Name Sodium-Potassium ATPase Subunit Beta 1, ATP1B, ATP1B1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CKAYGENIGYSEKDR, corresponding to amino acid residues 277 - 291 of mouse ATP1B1 (Accession P14094). Extracellular, C-terminus.
Accession (Uniprot) Number P14094
Gene ID 11931
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014), (1:500).
    3-4. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody, preincubated with Beta 1 Na+/K+ ATPase (extracellular) Blocking Peptide (BLP-NP014).
  • Western blot analysis of rat kidney membranes (lanes 1 and 3) and mouse heart membranes (lanes 2 and 4):
    Western blot analysis of rat kidney membranes (lanes 1 and 3) and mouse heart membranes (lanes 2 and 4):
    1-2. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014), (1:200).
    3-4. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody, preincubated with Beta 1 Na+/K+ ATPase (extracellular) Blocking Peptide (BLP-NP014).
  • Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 4), human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 5) and human Colo-205 colon carcinoma cell line lysates (lanes 3 and 6):
    Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 4), human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 5) and human Colo-205 colon carcinoma cell line lysates (lanes 3 and 6):
    1-3. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014), (1:200).
    4-6. Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody, preincubated with Beta 1 Na+/K+ ATPase (extracellular) Blocking Peptide (BLP-NP014).
Immunohistochemistry
  • Expression of Beta 1 Na+/K+ ATPase in rat cerebellum.
    Expression of Beta 1 Na+/K+ ATPase in rat cerebellum.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Beta 1 Na+/K+ ATPase immunoreactivity (green) appears in structures surrounding Purkinje cell soma (down pointing arrows) and in cone-shaped structures at the Purkinje axon initial segment (up pointing arrows). B. Pre-incubation of the antibody with Beta 1 Na+/K+ ATPase (extracellular) Blocking Peptide (BLP-NP014), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Bajaj S, Han J. (2019) Front Pharmacol., 10, 58.
  2. Li, Z. et al. (2015) Front. Cell Dev. Biol., 3, 66.
  3. Geering, K. (2008) Curr Opin Nephrol Hypertens., 17, 526.
  4. Hilbers, F. et al. (2016) Sci Rep 6, 20442.
Scientific background

Ion channels are multimeric proteins located in the plasma membrane that play a critical role in the regulation of cellular processes1. The gradient of sodium (Na+) and potassium (K+) across the plasma membrane is regulated by the Na+/K+-ATPase enzyme complex. Na/K-ATPase hydrolyzes one ATP molecule in order to pump three Na+ ions out of the cell and two K+ ions into the cell during every pumping cycle. Na+/K+-ATPase activity is vital for sodium and potassium homeostasis in cells, which are important for numerous enzymatic functions and secondary transport of different molecules2

Na+/K+-ATPase is a heteromeric transmembrane protein complex consisting of α- and β-subunits and a regulatory subunit belonging to the FXYD proteins. The catalytic α-subunit is responsible for transport activities and contain 10 transmembrane domains (TMDs). The β-subunit is heavily glycosylated and function as a molecular chaperone. The association of the β-subunit increases the stability of the catalytic α-subunit and dictates the affinity toward sodium and potassium. In addition, the β-subunit is necessary for the complex migration into the plasma membrane3.

β-subunits has three different isoforms β-1, β-2 and β-3 that consist of small N-terminal (30-AA), a TM helix and large C-terminal (240-AA) with different tissue specificities. β-1 found to have additional role in the response of cells to oxidative stress via glutathionylation of a cysteine residue located at the center of the TMD, the same cysteine is absent in the other beta subunits4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 29/09/2021

Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody (#ANP-014) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1B1 from rat, mouse and human samples.

For research purposes only, not for human use
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