Free shipping starts now, no minimum, no coupons required!

Anti-BGT-1 (SLC6A12) Antibody

Betaine/GABA transporter 1, Na+/Cl--dependent betaine/GABA transporter
Cat #: AGT-004
Alternative Name Betaine/GABA transporter 1, Na+/Cl--dependent betaine/GABA transporter
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)GEMMDQKDKDQVKD, corresponding to amino acid residues 22-35 of rat Betaine/GABA Transporter 1 (Accession P48056). Intracellular, N-terminus.
Accession (Uniprot) Number P48056
Gene ID 50676
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 13/14 amino acid residues identical.
RRID AB_2340978.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1,2. Anti-BGT-1 (SLC6A12) Antibody (#AGT-004), (1:200).
    3,4. Anti-BGT-1 (SLC6A12) Antibody, preincubated with BGT-1/SLC6A12 Blocking Peptide (#BLP-GT004).
  • Western blot analysis of mouse kidney membranes:
    Western blot analysis of mouse kidney membranes:
    1. Anti-BGT-1 (SLC6A12) Antibody (#AGT-004), (1:200).
    2. Anti-BGT-1 (SLC6A12) Antibody, preincubated with BGT-1/SLC6A12 Blocking Peptide (#BLP-GT004).
References
  1. Amara, S.G. et al. (1993) Ann. Rev. Neurosci. 16, 73.
  2. Kempson, S.A. et al. (2014) Front. Physiol. 5, 1.
  3. Zhou, Y. et al. (2012) Am. J. Physiol. 202, F316.
  4. Evans, J.E. et al. (1996) J. Comp. Neurol. 376, 431.
  5. Lever, M. and Slow, S. (2010) Clin. Biochem. 43, 732.
Scientific background

γ-aminobutyric acid (GABA) transporters (GATs) are essential regulators of the activity in the GABAergic system through their continuous uptake of the neurotransmitter from the synaptic cleft and extrasynaptic space. Four GABA transporters (GAT1, GAT2, GAT3 and BGT1) have been identified.  Betaine/GABA transporter (BGT1, SLC6A12) is a member of the Na+- and Cl-dependent neurotransmitter transporter gene family (SLC6) with a high homology to GAT1 (SLC6A1), GAT2 (SLC6A13) and GAT3 (SLC6A11). They have 12 hydrophobic transmembrane domains (TMDs), a large extracellular loop between TMD3 and 4 with multiple glycosylation sites, and intracellular N- and C-termini1,2.

BGT1 expression may vary among species.  In the dog, BGT1 mRNA is primarily detected in the kidney while the levels in the brain and liver are considerably lower. The highest BGT1 mRNA levels in the mouse are in the liver, while the highest levels in man are in the kidney followed by the liver3. BGT-1 is also present in the brain, but appears to be at low levels and mostly in the leptomeninges rather than in brain tissue proper4.

Homozygous BGT1-deficient mice have normal development and show seizure susceptibility indistinguishable from that in wild-type mice in a variety of seizure threshold models5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-BGT-1 (SLC6A12) Antibody (#AGT-004) is a highly specific antibody directed against an epitope of the rat Betaine/GABA transporter 1. The antibody can be used in western blot analysis. It has been designed to recognize BGT-1 from mouse and rat samples. The antibody is unlikely to recognize the protein from human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information