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Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody

BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor

Cat #: ABR-002
Alternative Name BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RSYHYSEVDTSMLH, corresponding to amino acid residues 287-300 of human BB2R (Accession P30550). 3rd extracellular loop.
Accession (Uniprot) Number P30550
Gene ID 2925
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, dog - identical.
RRID AB_2039748.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002), (1:500).
    3,4. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody, preincubated with Bombesin Receptor 2/GRPR (extracellular) Blocking Peptide (#BLP-BR002).
  • Western blot analysis of DU 145 (lanes 1 and 3) and PC-3 (lanes 2 and 4) human prostate carcinoma cell lines:
    Western blot analysis of DU 145 (lanes 1 and 3) and PC-3 (lanes 2 and 4) human prostate carcinoma cell lines:
    1,2. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002), (1:200).
    3,4. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody, preincubated with Bombesin Receptor 2/GRPR (extracellular) Blocking Peptide (#BLP-BR002).
Immunohistochemistry
  • Expression of Bombesin receptor 2 in human colon
    Expression of Bombesin receptor 2 in human colon
    Immunohistochemical staining of paraffin-embedded human colon using Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002), (1:50). (A and B) Human colon showing malignant growth. Staining is specific for epithelium-derived malignant cells. (C and D) Normal colon, staining is specific for absorptive epithelial cells in the crypts of Lieberkuhn. Histofine (pink) is used for the color reaction. Hematoxilin is used as the counterstain.
Live cell imaging / Immunocytochemistry
  • Expression of Bombesin receptor 2 in human HT-29 cells
    Expression of Bombesin receptor 2 in human HT-29 cells
    Cell surface detection of BB2 receptor in live intact human HT-29 (colorectal adenocarcinoma) cells. Cells were stained with Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002) (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody, showing surface expression of the BB2 receptor.
References
  1. Battey, J.F. et al. (1991) Proc Natl Acad Sci U.S.A. 88, 395.
  2. Kroog, G.S. et al.(1995) Med. Res. Rev. 15, 389.
  3. Ohki-Hamazaki, H. et al. (2005) Int. J. Dev. Biol. 49, 293.
  4. Reubi, J.C. (2003) Endocr. Rev. 24, 389.
Scientific background

Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP; a 27 amino acid homologue) and neuromedin B (NMB; a 10 amino acid homologue).

BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R.1 BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified.

All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.2

GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestinal tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes.2 In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation.3

Finally, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located in the 3rd extracellular loop of the human BB2 receptor (gastrin-releasing peptide receptor). Anti-Bombesin Receptor 2 (extracellular) (GRPR) Antibody (#ABR-002) can be used in western blot analysis, as well as immunocytochemical and immunohistochemical applications, and will recognize BB2R from human, rat, mouse, and dog samples.

For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Human adenocarcinoma sections (1:500).
    Lim, J.C. et al. (2015) Nucl. Med. Biol. 42, 131.

Specifications

Scientific Background

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