Overview
Type: Synthetic peptide
Form: Lyophilized powder
Bombesin Receptor 2/GRPR (extracellular) Blocking Peptide (#BLP-BR002) is the original antigen used for immunization during Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Bombesin Receptor 2/GRPR (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002), (1:500).
3,4. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody, preincubated with Bombesin Receptor 2/GRPR (extracellular) Blocking Peptide (#BLP-BR002).
Western blot analysis of DU 145 (lanes 1 and 3) and PC-3 (lanes 2 and 4) human prostate carcinoma cell lines:1,2. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002), (1:200).
3,4. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody, preincubated with Bombesin Receptor 2/GRPR (extracellular) Blocking Peptide (#BLP-BR002).
Expression of Bombesin receptor 2 in human colonImmunohistochemical staining of paraffin-embedded human colon using Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002), (1:50). (A and B) Human colon showing malignant growth. Staining is specific for epithelium-derived malignant cells. (C and D) Normal colon, staining is specific for absorptive epithelial cells in the crypts of Lieberkuhn. Histofine (pink) is used for the color reaction. Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)RSYHYSEVDTSMLH, corresponding to amino acid residues 287-300 of human BB2R (Accession P30550).
Accession (Uniprot) Number P30550
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
