Overview
- Peptide CRYNFDSSRSYDYWD, corresponding to amino acid residues 172 - 186 of mouse C3aR1 (Accession O09047). Extracellular, 2nd loop.
Cell surface detection of C3aR1 by direct flow cytometry in live intact mouse BV-2 microglia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-C3aR1 (extracellular)-FITC Antibody (#AAR-036-F), (5µg).
Cell surface detection of C3aR1 by direct flow cytometry in live intact mouse J774 macrophage cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-C3aR1 (extracellular)-FITC Antibody (#AAR-036-F), (5µg).
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The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.
Three different pathways are known to activate the complement. The C3 complement plays a key role in all three pathways leading to the activation of the complement. Produced in the liver, C3 is the most abundant complement protein in the serum. It is composed of two polypeptides, an α and β chain. Cleavage of C3 leads to the production of C3b and C3a1.
C3a binds to C3a receptor, a G-protein coupled receptor which couples to Gi upon activation. Like all other members of the superfamily, C3a receptor has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1. The receptor has two glycosylation sites, an unusually large extracellular loop with a sulfated tyrosine residue important for ligand recognition and binding2,3.
C3a receptor is expressed in cells of myeloid origin like neutrophils, macrophages and dendritic cells to name a few2. It is also detected in astrocytes from inflamed brain4, endothelial cells5 and activated human T cells6. mRNA of C3a receptor is detected in lung, liver, kidney, brain heart, muscle and testis2,7.
Anti-C3aR1 (extracellular) Antibody (#AAR-036) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize C3aR1 from mouse and rat samples.
The antibody will not recognize C3aR1 from human samples.
Anti-C3aR1 (extracellular)-FITC Antibody (#AAR-036-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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