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Anti-Human C5aR1 (extracellular) Antibody

C5a1 receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88

Cat #: AAR-032
Alternative Name C5a1 receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5a anaphylatoxin receptor (Accession P21730). Extracellular, N-terminus.
Accession (Uniprot) Number P21730
Gene ID 728
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only. Not recommended for use with rat and mouse samples.
RRID AB_2340888.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis  of Human K562 myelogenous leukemia (lanes 1 and 4), MEG-01 chronic myelogenous leukemia cell (lanes 2 and 5), and THP-1 acute monocytic leukemia (lanes 3 and 6) cell lysates:
    Western blot analysis  of Human K562 myelogenous leukemia (lanes 1 and 4), MEG-01 chronic myelogenous leukemia cell (lanes 2 and 5), and THP-1 acute monocytic leukemia (lanes 3 and 6) cell lysates:
    1-3. Anti-Human C5aR1 (extracellular) Antibody (#AAR-032), (1:200).
    4-6. Anti-Human C5aR1 (extracellular) Antibody, preincubated with Human C5aR1 (extracellular) Blocking Peptide (#BLP-AR032).
Indirect flow cytometry
  • Cell surface detection of C5aR1 in HL-60 intact living cells:
    Cell surface detection of C5aR1 in HL-60 intact living cells:
    ___ Unstained cells.
    ___ Cells + Anti-Human C5aR1 (extracellular) Antibody (#AAR-032), (10 µg/1x106 cells).
  • The blocking peptide is not suitable for this application.
References
  1. Haas, P.J. and van Srijp, J. (2007) Immun. Res. 37, 161.
  2. Marder, S.R. et al. (1985) J. Immunol. 134, 3325.
  3. Guo, R.F. and Ward, P.A. (2005) Annu. Rev. Immunol. 23, 821.
  4. Schumacher, W.A. et al. (1991) Agents Actions 34, 345.
  5. Laudes, I.J. et al. (2002) Am. J. Pathol. 160, 1867.
  6. Geva, A. et al. (2000) J. Biol. Chem. 275, 35393.
  7. Klco, J.M. et al. (2003) J. Biol. Chem. 278, 35345.
  8. Monk, P.N. et al. (2007) Br. J. Pharmacol. 152, 429.
  9. Huttenrauch, F. et al. (2005) J. Biol. Chem. 280, 37503.
  10. Ward, P.A. (2009) J. Mol. Med. 87, 375.
  11. Gerard, N.P. et al. (1989) J. Biol. Chem. 264, 1760.
  12. Werfel, T. et al. (1992) Blood 79, 152.
Scientific background

The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.

C5a is one of the most potent inflammatory peptides generated by complement activation. This peptide has many functions such as acting as a chemoattractant for neutrophils2,3. C5a acts as a vasodilator3,4 and activates the coagulation pathway3,5 just to name a few.

C5a exerts its numerous actions by binding to C5aR and C5L2. C5aR belongs to the superfamily of G-protein coupled receptors (GPCRs). Like all members, C5aR has seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. C5aR can form homodimers6-8 or heteromer with CCR5, chemokine receptor, for example8,9. Activation of C5aR by C5a binding causes Ca2+ mobilization10.

C5aR expression is detected in neutrophils, eisinophils, basophils and monocytes3,11,12. The receptor is also expressed in lung and liver13,14.

Blocking C5aR by peptides or organic small molecules is in the pipeline for treating chronic inflammatory disorders/diseases like arthritis, inflammatory bowel disease and lung/liver injuries8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Human C5aR1 (extracellular) Antibody (#AAR-032) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize C5aR from human samples only.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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