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Anti-Calnexin Antibody

Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90

Cat #: ACS-009
Alternative Name Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx (Accession P35565). Intracellular, C-terminus.
Accession (Uniprot) Number P35565
Gene ID 29144
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756580.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat heart membrane (lanes 1 and 5), mouse heart membrane (lanes 2 and 6), human breast adenocarcinoma (MCF7) lysate (lanes 3 and 7) and human ovary adenocarcinoma (OVCAR-3) cell line lysate (lanes 4 and 8):
    Western blot analysis of rat heart membrane (lanes 1 and 5), mouse heart membrane (lanes 2 and 6), human breast adenocarcinoma (MCF7) lysate (lanes 3 and 7) and human ovary adenocarcinoma (OVCAR-3) cell line lysate (lanes 4 and 8):
    1-4. Anti-Calnexin Antibody (#ACS-009), (1:400).
    5-8. Anti-Calnexin Antibody, preincubated with Calnexin Blocking Peptide (#BLP-CS009).
Immunohistochemistry
  • Expression of Calnexin in mouse hippocampus
    Expression of Calnexin in mouse hippocampus
    Immunohistochemical staining of mouse hippocampal CA1 region using Anti-Calnexin Antibody (#ACS-009), (1:200). A. Canx staining (green) appears in neurons (horizontal arrow) and astrocytes (vertical arrow). B. GFAP staining of astrocytic fibers (red). C. Merge of panels A and B demonstrates co-localization in some astrocytes. DAPI is used to stain nuclei (blue).
References
  1. Ho, S.C. et al. (1999) Mol. Immunol. 36, 1.
  2. Nagaya, N. et al. (1999) Receptors Channels. 6, 229.
  3. Manganas, L.N. and Trimmer, J.S. (2004) Biochem. Biophys. Res. Commun. 322, 577.
  4. Pind, S. et al. (1994) J. Biol. Chem. 269, 12784.
Scientific background

Calnexin is a type I transmembrane endoplasmic reticulum chaperone protein involved in the folding and assembly of numerous proteins. Calnexin is comprised of 537 amino acids with a large luminal domain consisting of 462 amino acids. Calnexin does not possess N-linked glycosylation sites and is localized to the endoplasmic reticulum by a –RKPRRE motif at its carboxyl terminus. Calnexin is one of the few ER membrane proteins that can be phosphorylated in a GTP-dependent manner1.

Calnexin interacts with a number of ER membrane channels. It interacts transiently with wild-type Shaker K+ channel in the ER but fails to associate with an unglycosylated Shaker mutant that makes active, cell surface channels. This suggest that glycosylation of Shaker protein is required for interaction with calnexin but calnexin is not required for the proper assembly and folding of Shaker channels2.

Coexpression of calnexin with the voltage-gated potassium channel KV1.2α was found to produce a substantial dose-dependent increase in cell surface KV1.2α channels in transfected mammalian COS-1 cells. In contrast, calnexin coexpression showed no effect on trafficking of intracellularly retained KV1.1 or KV1.6α subunits3.

Calnexin has been implicated in the pathophysiology of cystic fibrosis disease (CF). Wild type and ΔF508 mutant cystic-fibrosis conductance regulator (CFTR) associate with calnexin in a Chinese hamster ovary (CHO) cell model. Although both wild-type and ΔF508 CFTRs are present in complexes with calnexin, only wild-type CFTR is able to escape this association and exit the ER. This suggests that calnexin retains misfolded proteins in the ER and contributes to the mislocalization of mutant CFTRs.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Calnexin Antibody (#ACS-009) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Canx from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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