Anti-Cannabinoid Receptor 1 (extracellular) Antibody

    Cat #: ACR-001
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Peptide NKSLSSFKENEENIQC, corresponding to amino acid residues 84-99 of rat CB1 receptor (Accession P20272). Extracellular, N-terminus.
    Accession (Uniprot) Number P20272
    Gene ID 25248
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - identical; mouse - 15/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.7 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain membrane proteins:
    1. Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001), (1:200).
    2. Anti-Cannabinoid Receptor 1 (extracellular) Antibody, preincubated with the control peptide antigen.
    Expression of CB1R in mouse hippocampus
    Immunohistochemical staining of mouse hippocampus using Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001), (1:100). A. CB1R is detected in pyramidal and infra-pyramidal layers (green). B. Staining of interneurons using mouse anti-parvalbumin (PV) antibody (red). C. Confocal merge of A and B does not indicate the presence of CB1R in GABAergic cells.
    Expression of CB1R in rat hippocampus
    Immunohistochemical staining of rat hippocampus using Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001) (1:100). A. CB1R is detected in pyramidal and infra-pyramidal layers (green). B. Staining of interneurons using mouse anti-parvalbumin (PV) antibody (red). C. Confocal merge of A and B does not indicate the presence of CB1R in GABAergic cells.
    Live cell imaging / Immunocytochemistry
    Rat cultured hippocampal neurons (1:100 for live cells, 1:500-1:100 for fixed and permeabilized cells) (McDonald, N.A. et al. (2007) Mol. Pharmacol. 71, 976.).
    1. Brooks, J.W. and Fraquhar-Smith, M.A. (2003) Br. J. Anaesth3, 175.
    2. Howlett, A.C. (2002) Prostaglandins Other Lipid Mediat. 68–69, 619.
    3. Howlett, A.C. et al. (2002) Pharmacol. Rev54, 161.
    4. Rueda, D. et al. (2000) Mol. Pharmacol58, 814.
    5. Sarfaraz, S. et al. (2005) Cancer Res65, 1635.
    6. Casanova, M.L. et al. (2003) J. Clin. Invest111, 43.
    7. Cota, D. et al. (2003) Int. J. Obes. Relat. Metab. Disord27, 289.
    Scientific background

    Cannabinoids have been used in eastern medicine for many years as pain relievers.1 Δ9-tetrahydrocannabinol (THC), the major psychoactive compound in marijuana and hashish, has been shown to interact with two specific cannabinoid receptors: cannabinoid receptor 1 (CB1 Receptor or CB1R) and cannabinoid receptor 2 (CB2 Receptor or CB2R).2 The cannabinoid receptors can be distinguished by their amino acid sequences, signaling mechanisms, and tissue distributions.2 Both receptors belong to the G-protein-coupled receptor (GPCR) superfamily. CB1R was shown to modulate several Ca2+ and K+ ion channels.2,3

    CB1R is primarily expressed in the central nervous system. However, expression of CB1R is also detected in the peripheral terminals, in non-neuronal peripheral tissues such as uterus, testes, spleen, as well as in cells of the immune system.3,4

    CB1R is implicated in many cellular functions such as neurotransmitter release, pain relief, cancer, and obesity.5,6 Growth inhibition of tumor cells was demonstrated following mixed CB1R/CB2R agonist treatment in both prostate and non-melanoma skin cancers.5,6 Through their interaction with CB1R, cannabinoid compounds stimulate appetite for sweets and palatable foods in particular, making CB1R an attractive therapeutic target for the treatment of obesity and eating disorders.7

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Immuno-colocalization of proNGF and Cannabinoid Receptor 1 in mouse hippocampal dentate gyrus
    Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Guinea pig Anti-proNGF Antibody (#AGP-031), (1:300) and rabbit Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001), (1:300). A. proNGF staining (red) appears in the dentate gyrus (DG) granule layer (G) and in hilar interneurons (arrows). B. CB1R staining (green) appears in axonal processes surrounding the granule layer and a few hilar interneurons (arrows). C. Merge of the two images reveals co-localization of proNGF and CB1R in a few hilar cells. Cell nuclei were stained with DAPI (blue).

    Last update: 01/11/2018

    Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize CB1R in human, mouse, and rat samples.

    For research purposes only, not for human use
    Live cell imaging citations
    1. HEK 293 transfected cells (1:400).
      Hajkova, A. et al. (2016) Neuropharmacology 107, 201.
    Immunocytochemistry citations
    1. Rat embryonic cortical neurons (1:200).
      Hajkova, A. et al. (2016) Neuropharmacology 107, 201.
    More product citations
    1. Chen, S.W. et al. (2012) PloS ONE 7, e50850.
    2. Gonzalez Islas, C. et al. (2012) J. Neurosci. 32, 13597.
    3. McDonald, N.A. et al. (2007) Mol. Pharmacol. 71, 976.
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