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Anti-Cannabinoid Receptor 1 (extracellular) Antibody

CB1, CB-R, CNR1, CB1R, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor
Cat #: ACR-001
Alternative Name CB1, CB-R, CNR1, CB1R, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide NKSLSSFKENEENIQC, corresponding to amino acid residues 84-99 of rat CB1 receptor (Accession P20272). Extracellular, N-terminus.
Accession (Uniprot) Number P20272
Gene ID 25248
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - identical; mouse - 15/16 amino acid residues identical.
RRID AB_2039795.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.7 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain membrane proteins:
    Western blot analysis of rat brain membrane proteins:
    1. Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001), (1:200).
    2. Anti-Cannabinoid Receptor 1 (extracellular) Antibody, preincubated with Cannabinoid Receptor 1 (extracellular) Blocking Peptide (#BLP-CR001).
Immunohistochemistry
  • Expression of CB1 receptor in mouse hippocampus
    Expression of CB1 receptor in mouse hippocampus
    Immunohistochemical staining of mouse hippocampus using Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001), (1:100). A. CB1 is detected in pyramidal and infra-pyramidal layers (green). B. Staining of interneurons using mouse anti-parvalbumin (PV) antibody (red). C. Confocal merge of A and B does not indicate the presence of CB1 in GABAergic cells.
  • Expression of CB1 receptor in rat hippocampus
    Expression of CB1 receptor in rat hippocampus
    Immunohistochemical staining of rat hippocampus using Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001) (1:100). A. CB1 is detected in pyramidal and infra-pyramidal layers (green). B. Staining of interneurons using mouse anti-parvalbumin (PV) antibody (red). C. Confocal merge of A and B does not indicate the presence of CB1 in GABAergic cells.
Live cell imaging / Immunocytochemistry
  • Rat cultured hippocampal neurons (1:100 for live cells, 1:500-1:100 for fixed and permeabilized cells) (McDonald, N.A. et al. (2007) Mol. Pharmacol. 71, 976.).
References
  1. Brooks, J.W. and Fraquhar-Smith, M.A. (2003) Br. J. Anaesth. 3, 175.
  2. Howlett, A.C. (2002) Prostaglandins Other Lipid Mediat. 68–69, 619.
  3. Howlett, A.C. et al. (2002) Pharmacol. Rev. 54, 161.
  4. Rueda, D. et al. (2000) Mol. Pharmacol. 58, 814.
  5. Sarfaraz, S. et al. (2005) Cancer Res. 65, 1635.
  6. Casanova, M.L. et al. (2003) J. Clin. Invest. 111, 43.
  7. Cota, D. et al. (2003) Int. J. Obes. Relat. Metab. Disord. 27, 289.
Scientific background

Cannabinoids have been used in Eastern medicine for many years as pain relievers.1 Δ9-tetrahydrocannabinol (THC), the major psychoactive compound in marijuana and hashish, has been shown to interact with two specific cannabinoid receptors: cannabinoid receptor 1 (CB1 receptor) and cannabinoid receptor 2 (CB2 receptor).2 The cannabinoid receptors can be distinguished by their amino acid sequences, signaling mechanisms, and tissue distributions.2 Both receptors belong to the G-protein coupled receptor (GPCR) superfamily. CB1 was shown to modulate several Ca2+ and K+ ion channels.2,3

CB1 is primarily expressed in the central nervous system. However, expression of CB1 is also detected in the peripheral terminals, in non-neuronal peripheral tissues such as uterus, testes, spleen, as well as in cells of the immune system.3,4

CB1 is implicated in many cellular functions such as neurotransmitter release, pain relief, cancer, and obesity.5,6 Growth inhibition of tumor cells was demonstrated following mixed CB1/CB2 agonist treatment in both prostate and non-melanoma skin cancers.5,6 Through their interaction with CB1, cannabinoid compounds stimulate appetite for sweets and palatable foods in particular, making CB1 an attractive therapeutic target for the treatment of obesity and eating disorders.7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-Cannabinoid Receptor 1 (extracellular) Antibody
Multiplex staining of proNGF and Cannabinoid Receptor 1 in mouse hippocampal dentate gyrus
Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Guinea pig Anti-proNGF Antibody (#AGP-031), (1:300) and rabbit Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001), (1:300). A. proNGF staining (red) appears in the dentate gyrus (DG) granule layer (G) and in hilar interneurons (arrows). B. CB1 staining (green) appears in axonal processes surrounding the granule layer and a few hilar interneurons (arrows). C. Merge of the two images reveals co-localization of proNGF and CB1 in a few hilar cells. Cell nuclei were stained with DAPI (blue).

Last update: 16/08/2020

Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001) is a highly specific antibody directed against an epitope of the rat CB1 receptor. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize CB1 in human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Live cell imaging citations
  1. HEK 293 transfected cells (1:400).
    Hajkova, A. et al. (2016) Neuropharmacology 107, 201.
Immunohistochemistry citations
  1. Human kidney sections.
    Udi, S. et al. (2010) Br. J. Pharmacol. 177, 110.
Immunocytochemistry citations
  1. Rat embryonic cortical neurons (1:200).
    Hajkova, A. et al. (2016) Neuropharmacology 107, 201.
More product citations
  1. Chen, S.W. et al. (2012) PloS ONE 7, e50850.
  2. Gonzalez Islas, C. et al. (2012) J. Neurosci. 32, 13597.
  3. McDonald, N.A. et al. (2007) Mol. Pharmacol. 71, 976.
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