Anti-CaV1.1 (CACNA1S) (extracellular) Antibody

Voltage-dependent L-type calcium channel subunit α1S, CACH1, CACN1, CACH1B, CACNL1A3
    Cat #: ACC-314
    Alternative Name Voltage-dependent L-type calcium channel subunit α1S, CACH1, CACN1, CACH1B, CACNL1A3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CGNVDPDESARIS, corresponding to amino acid residues 506-518 of rat CaV1.1 (Accession Q02485). 2nd extracellular loop, domain IV.
    • Anti-CaV1.1 (CACNA1S) (extracellular) Antibody
    Accession (Uniprot) Number Q02485
    Gene ID 682930
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_2340894.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-CaV1.1 (CACNA1S) (extracellular) Antibody
      Western blot analysis of rat skeletal muscle:
      1. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:200).
      2. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-CaV1.1 (CACNA1S) (extracellular) Antibody
      Expression of CaV1.1 in rat skeletal muscle
      Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:100), followed by DAB. CACNA1S staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.
    References
    1. Zuccotti, A. et al. (2011) Trends Pharmacol. Sci. 32, 366.
    2. Tanabe, T. et al. (1987) Nature 328, 313.
    3. Bannister, R.A. and Beam, K.G. (2013) Biochim. Biophys. Acta 1828, 1587.
    4. Stroffekova, K. (2008) Pflugers Arch. 455, 873.
    5. Renner, D.R. and Ptacek, L.J. (2002) Adv. Neurol. 88, 235.
    6. Jurkat-Rott, K. Lehmann-Horn, F. (2005) J. Clin. Invest. 115, 2000.
    Scientific background

    Voltage-gated Ca2+ channels (VGCCs) serve as key transducers coupling changes in cell surface membrane potential with local intracellular Ca2+ pathways. Among the three families of VGCCs, L-type Ca2+ channels (L-VGCCs), include four different isoforms of the α1 pore-forming subunit: CaV1.1, CaV1.2CaV1.3 and CaV1.41.

    CaV1.1 (α1S) is the prototypical VGCCs. It was the first member of the CaV family to be cloned2. CaV1.1 is a single polypeptide composed of four relatively conserved repeats (I, II, II and IV) containing six α-helices a piece. The fourth α-helix of each repeat has a regularly spaced sequence of basic residues that is believed to be critical for voltage-sensing. The I–II-linker is the site of interaction with the intracellular β1a subunit3.

    CaV1.1 is expressed solely in skeletal muscle. It is localized in regions of the T-tubular membrane that are closely opposed to the sarcoplasmic reticulum (the TSR junction). The primary role of CaV1.1 is to serve as the voltage sensor for skeletal muscle-type excitation contraction (EC) coupling4.

    Mutations in CaV1.1 have been identified as causative for two congenital muscle pathophysiologies: hypokalemic periodic paralysis5 (HypoKPP) and malignant hyperthermia6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat CaV1.1 channel. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize CaV1.1 from rat, mouse and human samples.

    For research purposes only, not for human use
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