- Peptide CGNVDPDESARIS, corresponding to amino acid residues 506-518 of rat CaV1.1 (Accession Q02485). 2nd extracellular loop, domain IV.
- Western blot analysis of rat skeletal muscle:1. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:200).
2. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody, preincubated with Cav1.1/CACNA1S (extracellular) Blocking Peptide (#BLP-CC314).
- Expression of CaV1.1 in rat skeletal muscleImmunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314), (1:100), followed by DAB. CACNA1S staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.
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Voltage-gated Ca2+ channels (VGCCs) serve as key transducers coupling changes in cell surface membrane potential with local intracellular Ca2+ pathways. Among the three families of VGCCs, L-type Ca2+ channels (L-VGCCs), include four different isoforms of the α1 pore-forming subunit: CaV1.1, CaV1.2, CaV1.3 and CaV1.41.
CaV1.1 (α1S) is the prototypical VGCCs. It was the first member of the CaV family to be cloned2. CaV1.1 is a single polypeptide composed of four relatively conserved repeats (I, II, II and IV) containing six α-helices a piece. The fourth α-helix of each repeat has a regularly spaced sequence of basic residues that is believed to be critical for voltage-sensing. The I–II-linker is the site of interaction with the intracellular β1a subunit3.
CaV1.1 is expressed solely in skeletal muscle. It is localized in regions of the T-tubular membrane that are closely opposed to the sarcoplasmic reticulum (the TSR junction). The primary role of CaV1.1 is to serve as the voltage sensor for skeletal muscle-type excitation contraction (EC) coupling4.
Mutations in CaV1.1 have been identified as causative for two congenital muscle pathophysiologies: hypokalemic periodic paralysis5 (HypoKPP) and malignant hyperthermia6.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat CaV1.1 channel. Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize CaV1.1 from rat, mouse and human samples.