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Anti-CACNG1 (extracellular) Antibody

CaVγ1, CACNLG, Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle Ca2+ channel subunit gamma

Cat #: ACC-111
Alternative Name CaVγ1, CACNLG, Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle Ca2+ channel subunit gamma
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)DHWAVLSPHLEHHNE, corresponding to amino acid residues 30-44 of rat CACNG1 (Accession P97707). 1st extracellular loop.
Accession (Uniprot) Number P97707
Gene ID 29658
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 13/14 amino acid residues identical.
RRID AB_2756549.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat skeletal muscle lysate:
    Western blot analysis of rat skeletal muscle lysate:
    1. Anti-CACNG1 (extracellular) Antibody (#ACC-111), (1:400).
    2. Anti-CACNG1 (extracellular) Antibody, preincubated with CACNG1 (extracellular) Blocking Peptide (#BLP-CC111).
Immunohistochemistry
  • Expression of CaVγ1 (CACNG1) in rat skeletal muscle
    Expression of CaVγ1 (CACNG1) in rat skeletal muscle
    Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CACNG1 (extracellular) Antibody (#ACC-111), (1:100), followed by DAB. CaVγ1 staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.
References
  1. Catterall, W.A. (2000) Annu. Rev. Cell. Dev. Biol. 16, 521.
  2. Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
  3. De Jongh, K.S. et al. (1990) J. Biol. Chem. 265, 14738.
  4. Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.
  5. Lacinova, L. et al. (2005) Gen. Physiol. Biophys. 1, 1.
  6. Ursu, D. et al. (2004) J. Gen. Physiol. 124, 605.
Scientific background

Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+conducting pores in the plasma membrane1. On the basis of their voltage activation properties, the voltage-gated Ca2+ channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels.2. CaV channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3.

CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. The γ subunit is an integral membrane protein. The γ subunit family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5.

CaVγ1 is solely expressed in skeletal muscle and associates with the α1S (CaV1.1) subunit6.

Knockout mice are indeed viable and show no extreme phenotype. It seems that γ1 enhances the inactivation of ER Ca2+ release in a voltage-dependent manner6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaVγ1. Anti-CACNG1 (extracellular) Antibody (#ACC-111) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize CaVγ1 from rat, mouse and human samples.

For research purposes only, not for human use
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