- Peptide (C)DHWAVLSPHLEHHNE, corresponding to amino acid residues 30-44 of rat CACNG1 (Accession P97707). 1st extracellular loop.
- Western blot analysis of rat skeletal muscle lysate:1. Anti-CACNG1 (extracellular) Antibody (#ACC-111), (1:400).
2. Anti-CACNG1 (extracellular) Antibody, preincubated with CACNG1 (extracellular) Blocking Peptide (#BLP-CC111).
- Expression of CaVγ1 (CACNG1) in rat skeletal muscleImmunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-CACNG1 (extracellular) Antibody (#ACC-111), (1:100), followed by DAB. CaVγ1 staining is highly specific for striated muscle cells. Note that smooth muscle cells of arteries are not stained (arrow). Hematoxilin is used as the counterstain.
- Catterall, W.A. (2000) Annu. Rev. Cell. Dev. Biol. 16, 521.
- Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
- De Jongh, K.S. et al. (1990) J. Biol. Chem. 265, 14738.
- Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.
- Lacinova, L. et al. (2005) Gen. Physiol. Biophys. 1, 1.
- Ursu, D. et al. (2004) J. Gen. Physiol. 124, 605.
Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+conducting pores in the plasma membrane1. On the basis of their voltage activation properties, the voltage-gated Ca2+ channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels.2. CaV channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3.
CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. The γ subunit is an integral membrane protein. The γ subunit family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5.
CaVγ1 is solely expressed in skeletal muscle and associates with the α1S (CaV1.1) subunit6.
Knockout mice are indeed viable and show no extreme phenotype. It seems that γ1 enhances the inactivation of ER Ca2+ release in a voltage-dependent manner6.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaVγ1. Anti-CACNG1 (extracellular) Antibody (#ACC-111) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize CaVγ1 from rat, mouse and human samples.
- Anti-CaV1.1 (CACNA1S) (extracellular) Antibody (#ACC-314)
- Anti-Stargazin Antibody (#ACC-012)
- Anti-CACNG3 Antibody (#ACC-113)
- Anti-CACNG4 Antibody (#ACC-114)
- Anti-CACNG5 (extracellular) Antibody (#ACC-115)
- Anti-CACNG6 (extracellular) Antibody (#ACC-112)
- Anti-CACNG7 (extracellular) Antibody (#ACC-109)
- Anti-CACNG8 Antibody (#ACC-125)