Anti-CACNG3 Antibody

CaVγ3, Voltage-dependent calcium channel gamma-3 subunit, TARP gamma-3, Transmembrane AMPAR regulatory protein gamma-3
Cat #: ACC-113
Alternative Name CaVγ3, Voltage-dependent calcium channel gamma-3 subunit, TARP gamma-3, Transmembrane AMPAR regulatory protein gamma-3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RSHSELLKKSTFAR, corresponding to amino acid residues 210-223 of rat CACNG3 (Accession Q8VHX0). Intracellular, C-terminus.
    Accession (Uniprot) Number Q8VHX0
    Gene ID 140724
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical, human - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip
      • Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) cells (lanes 3 and 6):
        1-3. Anti-CACNG3 Antibody (#ACC-113), (1:1000).
        4-6. Anti-CACNG3 Antibody, preincubated with the control peptide antigen.
      • Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, Ca2+ channels have traditionally been classified into L, T, N, P/Q, and R types2.

        L-type calcium channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the subunits α2δ, γ, and β3.

        The γ subunit is an integral membrane protein. The γ family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5. CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. CaVγ3 mRNAs are only detectable in mouse brain6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TARP gamma-3 (CaVγ3, CACNG3). Anti-CACNG3 Antibody (#ACC-113) can be used in western blot analysis. It has been designed to recognize TARP gamma-3 from rat, mouse and human samples.

    For research purposes only, not for human use