Anti-CaVγ5 (extracellular) Antibody

Voltage-dependent calcium channel γ-5 subunit, Transmembrane AMPAR Regulatory Protein γ-5, TARP γ-5, TARP Gamma-5
    Cat #: ACC-115
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)SESTVNVLKMIRS, corresponding to amino acid residues 87-99 of rat CaVγ5 (Accession Q8VHW8). 1st extracellular loop.
    Accession (Uniprot) Number Q8VHW8
    Gene ID 140726
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    1, 2. Anti-CaVγ5 (extracellular) Antibody (#ACC-115), (1:1000).
    3, 4. Anti-CaVγ5 (extracellular) Antibody preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of CaVγ5 in living rat PC12 cells
    Immunocytochemical staining of intact living rat PC12 cells using Anti-CaVγ5 (extracellular) Antibody (#ACC-115), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Extracellular staining (red) merged with live view of the cells. B. Extracellular staining merged with nuclear staining using DAPI as the counterstain (blue).
    References
    1. Catterall, W.A. (2000) Annu. Rev. Cell. Dev. Biol16, 521.
    2. Qin, N. et al. (2002) Mol. Pharmacol62, 485.
    3. De Jongh, K.S. et al. (1990)   J. Biol. Chem. 265, 14738.
    4. Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.
    5. Lacinova, L. et al. (2005) Gen. Physiol. Biophys1, 1.
    6. Klugbauer N. et al. (2000) FEBS Lett. 470,189.
    7. Burgess, D.L. et al. (2009) Genome Res. 9, 1204.
    Scientific background

    Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. On the basis of their voltage activation properties, the voltage-gated Ca2+ channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels.2. CaV channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3.

    CaVγ subunits inhibit CaV channel activity and modulate its activation and inactivation kinetics. CaVγ subunits have little effect on CaV channel trafficking4. The γ subunit is an integral membrane protein. The γ subunit family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues5.

    The CaVγ5 subunit is highly expressed in the liver, kidney, heart, lung, skeletal muscles, and, with a lower abundance, in testes5. The close association of epilepsy and ataxia with mutations in other neuronal voltage-dependent Ca2+ channels suggests these are potential candidate phenotypes for defects in the CaVγ5 (CACNG5) gene6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat CaVγ5. Anti-CaVγ5 (extracellular) Antibody (#ACC-115) can be used in western blot and live cell imaging experiments. It has been designed to recognize CaVγ5 from rat, mouse and human samples.

    For research purposes only, not for human use