Anti-CLC-5 Antibody

CLCN5, Chloride channel 5, Chloride transporter ClC-5, H+/Cl- exchange transporter 5, DENTS
    Cat #: ACL-003
    Alternative Name CLCN5, Chloride channel 5, Chloride transporter ClC-5, H+/Cl- exchange transporter 5, DENTS
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide CDYENHFNTSKGGEL, corresponding to amino acid residues 401-415 of rat CLCN5 (Accession P51796). Extracellular.
    Accession (Uniprot) Number P51796
    Gene ID 25749
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 14/15 amino acid residues identical.
    RRID AB_2039816.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
      • Anti-CLC-5 Antibody
        Western blot analysis of rat kidney lysate:
        1. Anti-CLC-5 Antibody (#ACL-003), (1:200).
        2. Anti-CLC-5 Antibody, preincubated with the control peptide antigen.
      • Rat kidney sections.
    References
      • CLC5 is a member of the voltage-dependent Cl- channel family that includes nine known members. Based on sequence similarity, CLC5 is closely linked to the CLC3 and CLC4 subunits.  

        CLC-5 is mainly found in intracellular vesicles, particularly in early endosomes, where it is believed to contribute to their acidification.

        The channel is mainly expressed in the kidney, although it has also been found in the small intestine, brain and liver.

        Mutations in the CLC5 gene cause Dent's disease, an X-linked renal disorder.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 31/07/2019

    Anti-CLC-5 Antibody (#ACL-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CLC-5 from human, mouse, and rat samples.

    For research purposes only, not for human use