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Anti-SLC28A1 (CNT1) Antibody

Concentrative nucleoside transporter 1, Sodium/nucleoside cotransporter 1

Cat #: ANT-061
Alternative Name Concentrative nucleoside transporter 1, Sodium/nucleoside cotransporter 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)ENMGAEFLESMEEGR, corresponding to amino acid residues 22-36 of rat SLC28A1 (Accession Q62674). Intracellular, N-terminus.
Accession (Uniprot) Number Q62674
Gene ID 116642
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 13/15 amino acid residues identical; human - 12/15 amino acid residues identical.
RRID AB_2341016.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 4), rat liver (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:
    Western blot analysis of rat kidney (lanes 1 and 4), rat liver (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:
    1-3. Anti-SLC28A1 (CNT1) Antibody (#ANT-061), (1:200).
    4-6. Anti-SLC28A1 (CNT1) Antibody, preincubated with SLC28A1/CNT1 Blocking Peptide (#BLP-NT061).
References
  1. Molina-Arcas, M. et al. (2009) Curr. Vasc. Pharmacol. 7, 426.
  2. Rose, J.B. and Coe, I.R. (2008) Physiology 23, 41.
  3. Baldwin, S.A. et al. (2004) Pflugers Arch. 447, 735.
  4. Gray, J.H. et al. (2004) Pflugers Arch. 447, 728.
  5. Ritzel, M.W. et al. (1998) Mol. Membr. Biol. 15, 203.
  6. Ritzel, M.W. et al. (2001) J. Biol. Chem. 276, 2914.
  7. Smith, K.M. et al. (2005) J. Biol. Chem. 280, 25436.
  8. Pennycooke, M. et al. (2001) Biochem. Biophys. Res. Commun. 280, 951.
  9. Felipe, A. et al. (1998) Biochem. J. 330 (Pt.2), 997.
  10. Valdes, R. et al. (2000) Gastroenterology 119, 1623.
  11. Anderson, C.M. et al. (1996) Brain Res. Mol. Brain Res. 42, 358.
  12. Guillen-Gomez, E. et al. (2004) J. Neurochem. 90, 883.
Scientific background

Nucleosides play other important roles beyond their nucleic acid synthesis building block role. For example, they are involved in energy metabolism; they serve as ligands of purinergic receptors and act as influential signaling molecules1. Being hydrophilic, nucleosides cannot simply diffuse across the plasma membrane in order to exert their various functions, but rather need to be physically transported via nucleoside transporters1,2.

Two different transporter families are responsible for transporting nucleosides across the plasma membrane: the Concentrative nucleoside transporter proteins (CNT, SLC28 family), which consist of three members, CNT1-3, and act as Na+-dependent symporters1,3 and the Equilibrative nucleoside transporter proteins ENT1-4 (ENT, SLC29 family), which mediate a Na+-independent facilitated diffusion. Therefore, ENTs act as bidirectional carriers, responsible for the influx and efflux of substrates1.

CNTs are responsible for the intracellular uptake of nucleosides, an energy consuming process which is coupled to the Na+ gradient across the plasma membrane. All three transporters display a relatively high affinity for their substrates but a more selective than ENTs1,4. Pyrimidines are the substrate of choice for CNT1 although it can bind but not transport adenosine. CNT2 prefers purines, although it can transport uridine, and CNT3 is selective for both types of nucleosides. Both CNT1 and CNT2 display a 1:1 stoichiometry (nucleoside:sodium)1,5. CNT3 on the other hand transports two Na+ ions per nucleoside, and can also co-transport protons in a pH dependent manner in a 1:1 ratio1,6,7.

All three CNTs have thirteen transmembrane domains and an extracellular C-terminus responsible for substrate recognition1.

CNT1 is widely expressed in the small intestine, kidney and liver1,8-10. CNT2 is expressed in immune system cells1,8 and CNT3 expression is abundant in monocytes and macrophages. All three are detected in various regions of the brain1,6,11,12.

There is no evidence that nucleoside transporters are directly involved in pathophysiologies, but they are clinically significant. For example, nucleoside transporters are responsible for the cellular uptake of a number of nucleoside-derived anticancer drugs1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-SLC28A1 (CNT1) Antibody (#ANT-061) is a highly specific antibody directed against an epitope of the rat Concentrative nucleoside transporter 1. The antibody can be used in western blot analysis. It has been designed to recognize CNT1 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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