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Anti-Connexin-40 (GJA5) Antibody

CX40, Gap junction α5 protein

Cat #: ACC-205
Alternative Name CX40, Gap junction α5 protein
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)GHRFPQGYHSDKR, corresponding to amino acid residues 328-340 of rat Connexin-40 (Accession P28234). Intracellular, C-terminus.
Accession (Uniprot) Number P28234
Gene ID 50563
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
RRID AB_2340916.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart lysates:
    1,2. Anti-Connexin-40 (GJA5) Antibody (#ACC-205), (1:200).
    3,4. Anti-Connexin-40 (GJA5) Antibody, preincubated with Connexin-40/GJA5 Blocking Peptide (#BLP-CC205).
References
  1. Beyer, E.C. et al. 1993. Int. Rev. Cytol. 137C, 1.
  2. Evans, W.H. et al. 2002. Mol. Membr. Biol. 19, 121.
  3. Kelsell, D.P. et al. 2001. Trends Cell Biol. 11, 2.
  4. Bierhuizen, M.F. et al. 2000. Research 46, 511.
  5. Dupont, E. et al. 2001. J. Mol. Cell Cardiol. 33, 359.
  6. Miquerol, L. et al. 2003. Novartis Found Symp. 250, 80.
Scientific background

Connexins are transmembrane proteins, which oligomerize into hexameres adjusting in hemi-channels (connexons). The docking of connexons between two neighboring cells results in the formation of intercellular channels, which are permeable to ions and small molecules1. These channels, which are also known as gap junctions, are characterized by distinct electrophysiological properties. Various physiological and pathological conditions regulate the opening and closing of the channels2. Several genetic disorders have been identified in which specific connexin genes are mutated3.

Connexin 40 (Cx40) gene consists two exons, a small untranslated exon, exon 1, and an uninterrupted coding region located in an exon 24. In human and mouse species, Cx40 is expressed in various tissues and cells, such as choriocarcinoma cell lines, tonsil lymphocytes and cardiomyocytes5. The expression of the Cx40 gene in cardiomyocytes suggested that in the adult heart, Cx40 involved in the propagation of the electrical impulses from the atria to the ventricles. It was supported by the finding that Cx40 knockout mice developed cardiac conduction defects which matched the pattern of expression of the gene6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Anti-Connexin-40 (GJA5) Antibody (#ACC-205) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Cx40 from mouse, rat, and human samples.

For research purposes only, not for human use
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