Anti-Connexin-43 Antibody

CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein
Cat #: ACC-201
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302). Intracellular, C-terminus.
    Accession (Uniprot) Number P17302
    Gene ID 2697
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of mouse brain membranes:
    1. Anti-Connexin-43 Antibody (#ACC-201), (1:400).
    2. Anti-Connexin-43 Antibody, preincubated with the control peptide antigen.
    Western blot analysis of rat heart membranes:
    1. Anti-Connexin-43 Antibody (#ACC-201), (1:400).
    2. Anti-Connexin-43 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Connexin-43 in rat cerebellum
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Connexin-43 Antibody (#ACC-201), (1:300). Cx43 staining (red) appeared in Bergmann glial fibers (arrows) in the molecular layer (MOL) and in the granule layer (G). Cell nuclei were stained with DAPI (blue).
    Human fetal cochlear sections (Locher, H. et al. (2015) Dev. Neurobiol. 75, 1219.).
    Immunocytochemistry
    Expression of Connexin-43 in rat intestinal epithelial IEC-6 cells
    Immunocytochemical staining of fixed and permeabilized rat intestinal epithelial IEC-6 cells. Cells were stained with Anti-Connexin-43 Antibody (#ACC-201), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Cell nuclei were visualized using Hoechst 33342 (blue).
    References
    1. Goodenough, D.A. and Paul, D.L. (2009) Cold Spring Harb. Persp. Biol. 1, a002576.
    2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423.
    3. Chew, S.S. et al. (2010) Exp. Neurol. 225, 250.
    4. Trexler, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836.
    5. Hu, X. et al. (2006) Biophys. J. 90, 140.
    6. Tang, Q. et al. (2009) J. Gen. Physiol. 133, 555.
    Scientific background

    Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. For example, Connexin-43 (Cx43) protein level is regulated by its turnover rate and by phosphorylation of various residues which ultimately determines its activity rate1.

    Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.

    Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases, deafness, and developmental abnormalities2,5,6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/03/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Connexin-43. Anti-Connexin-43 Antibody (#ACC-201) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize Cx43 from human, rat and mouse samples.

    For research purposes only, not for human use