Anti-Contactin-1/CNTN1 (extracellular) Antibody

Neural cell surface protein F3, Glycoprotein GP135, CNTN1
  • New
Cat #: ANR-171
Alternative Name Neural cell surface protein F3, Glycoprotein GP135, CNTN1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)RPDGQHDGKLFSTHK, corresponding to amino acid residues 947-961 of rat Contactin-1 (Accession Q63198). Extracellular, N-terminus.
    • Anti-Contactin-1/CNTN1 (extracellular) Antibody
    Accession (Uniprot) Number Q63198
    Gene ID 117258
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 14/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-Contactin-1/CNTN1 (extracellular) Antibody
      Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
      1,2. Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171), (1:200).
      3,4. Anti-Contactin-1/CNTN1 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Contactin-1/CNTN1 (extracellular) Antibody
      Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
      1.Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171), (1:400).
      2. Anti-Contactin-1/CNTN1 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Contactin-1/CNTN1 (extracellular) Antibody
      Expression of Contactin-1 in rat cerebral ventricle:
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Contactin-1 immunoreactivity (green) appears in ependymal cells (arrows) lining the lateral cerebral ventricle (LV). Cell nuclei are stained with DAPI (blue).
    References
    1. Gulisano, W. et al. (2017) Mol. Cell. Neurosci. 81, 64.
    2. Chen, D.H. et al. (2015) World J. Gastroenterol. 21, 9707.
    3. Liu, P. et al. (2012) Jpn. J. Clin. Oncol. 42, 612.
    Scientific background

    Contactin-1 (CNTN1), also known as F3/Contactin a cell adhesion molecule, is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein involved in neuronal development, synaptic maintenance and organization of neuronal networks. CNTNs are a subpopulation of molecules belonging to the immunoglobulin (Ig) superfamily, which includes six members: CNTN1, TAG-1/CNTN2, BIG-1/CNTN3, BIG-2/CNTN4, NB-2/CNTN5 and NB-3/CNTN61,2.

    In mammals, contactin-1 is mostly localized in the peripheral and central neural system. It is highly expressed at the axonal level and mediates various functions in both glial and neuronal cells; it is involved in neuronal and glial development and differentiation, myelination, synaptogenesis and synaptic function1.

    Contactin-1 expression profile differs in various brain regions and, within each area, it changes depending upon the cell type. For example, in the cerebellar cortex contactin-1 is predominant in granule cells in earlier developmental stages, whereas its activation is delayed in Purkinje neurons. In the hippocampus, it is detected on pyramidal neurons of CA1-CA3 fields and in granule layers of Dentate Gyrus (DG) where its expression persists in postnatally developed neurons. Hippocampal contactin-1 is highly expressed in the developing hippocampus, it progressively increases during postnatal life, but it declines during aging, paralleling cognitive impairment. This suggests that contactin-1 might play a role in memory processes, in line with studies indicating its involvement in neurotransmitter release and some forms of plasticity1.

    The role of contactin-1 in neurotransmitter release has been linked with its capability to regulate synaptic vesicle exocytosis. Contactin-1 overexpression had been shown to induce an increase of cAMP-Responsive Element Binding (CREB) phosphorylation, enhanced hippocampal-dependent LTP and memory especially in 12-months-old mice, where neurogenesis is particularly evident. Also, contactin-1 overexpression counteracts apoptosis, and induces an increase of the antiapoptotic- and neuroplasticity-pathway BDNF/CREB/Bcl-21,3.

    Contactin-1 plays an important role in cancer progression. The expression of contactin-1 is upregulated in primary lesions, and its expression level correlates with tumor metastasis in cancer patients. Studies have demonstrated that contactin-1 also plays a key role in diseases not related to the nervous system, and its most notable function is its participation in cancer progression; in cancers such as esophageal squamous cell carcinoma (ESCC), GC, lung adenocarcinoma, oral squamous cell carcinoma (OSCC), hepatocellular carcinoma and prostate cancer2,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 25/02/2020

    Anti-Contactin-1/CNTN1 (extracellular) Antibody (#ANR-171) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used western blot and immunohistochemistry applications. It has been designed to recognize CNTN1 from rat, mouse, and human samples.

    For research purposes only, not for human use
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