- Peptide (C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin (Accession Q60598). Intracellular.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1, 2. Anti-Cortactin Antibody (#APZ-018), (1:1000).
3, 4. Anti-Cortactin Antibody, preincubated with Cortactin Blocking Peptide (#BLP-PZ018).
- Western blot analysis of human K562 erythroleukemia cell line lysate (lanes 1 and 5), human HeLa cervical adenocarcinoma cell line lysate (lanes 2 and 6), human U-87MG glioblastoma cell line lysate (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 4 and 8):1-4. Anti-Cortactin Antibody (#APZ-018), (1:200).
5-8. Anti-Cortactin Antibody, preincubated with Cortactin Blocking Peptide (#BLP-PZ018).
- Expression of Cortactin in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Cortactin Antibody (#APZ-018), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA3 region, showed immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with Cortactin Blocking Peptide (BLP-PZ018), suppressed staining. Cell nuclei are stained with DAPI (blue). P= pyramidal layer, SR = stratum radiatum.
Cortical acting binding protein, Cortactin, is an actin-binding protein involved in cytoskeletal regulation1. Cortactin is a 63–65 kDa protein that consists of four major domains: N-terminal acidic (NTA), 6.5 tandems repeat, proline-rich domain, and the C-terminal Src homology 3 domains (SH3 domains)2 and it is regulated by post translational modifications such as phosphorylation and acetylation1.
The actin filament network provides mechanical support as well as protrusive and contractile forces essential for directed cell motility and cell and tissue morphogenesis. Cortactin activates the Arp2/3 complex, the main regulator of actin polymerization, and thereby regulates the assembly of branched actin3.
Overexpression of cortactin was shown to enhance cell migration, invasion, and tumor cell metastasis. It is also overexpressed in various types of cancer such as breast cancers as well as head and neck tumors1.
Species reactivity key:
Anti-Cortactin Antibody (#APZ-018) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cortactin from rat, mouse, and human samples.