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Anti-CRACR2A (EFCAB4B) Antibody

CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B

Cat #: ACC-324
Alternative Name CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
Immunogen
  • Peptide (C)KSEKEQFLLKDTER, corresponding to amino acid residues 245-258 of human CRACR2A (Accession Q9BSW2). Intracellular.
Accession (Uniprot) Number Q9BSW2
Gene ID 84766
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 13/14 amino acid residues identical.
RRID AB_2756558.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of human T cell leukemia (Jurkat) (lanes 1 and 3) and rat stomach (lanes 2 and 4) lysates:
    Western blot analysis of human T cell leukemia (Jurkat) (lanes 1 and 3) and rat stomach (lanes 2 and 4) lysates:
    1,2. Anti-CRACR2A (EFCAB4B) Antibody (#ACC-324), (1:400).
    3,4. Anti-CRACR2A (EFCAB4B) Antibody, preincubated with CRACR2A/EFCAB4B Blocking Peptide (#BLP-CC324).
References
  1. Vig, M. and Kinet, J.P. (2007) Cell Calcium. 42, 157.
  2. Srikanth, S. et al. (2010) Nat. Cell Biol. 12, 436.
Scientific background

Calcium is a vital ion for the functioning of many intracellular processes. When Ca2+ stored in the endoplasmic reticulum is depleted, there is an activation of store-operated channels that results in the influx of Ca2+ from outside the cell. In non-excitable cells and especially in immune cells, most of this influx is carried out by calcium release activated calcium channels also known as “CRACs”1.

Mediation of signals from the ER to CRACs and activation of the CRAC channel is conducted by two modulators that physically cluster with the CRAC channel known as Orai1 and STIM1. STIM1 is a single transmembrane segment protein that has a long C-terminal cytoplasmic region and an N-terminus which detects ER Ca2+ depletion. Orai1 is a macromolecular complex with linkers of varying lengths between the ER and plasma membranes.

Novel findings suggest the existence of a complex comprised of Orai1, STIM1 and additional proteins. One of these proteins is an EF-hand protein named CRACR2A which regulates Orai-STIM interaction. An EF-hand is a structural domain found in Ca2+ binding proteins consisting of two alpha helices linked by a short loop region.

CRACR2A interacts with the cytoplasmic regions of both Orai1 and STIM1 thus creating a tertiary complex. Under high concentrations of Ca2+, CRACR2A caused a dissociation of Orai1 and STIM1 and a mutated CRACR2A was found, in one study, to cause elevated Ca2+ levels that caused cell death in T cells2.

Further research is required to fully determine the activation and regulation mechanisms of CRACs and this can be performed by better understanding CRACR2A and its binding proteins.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human CRAC channel regulator 2A. Anti-CRACR2A (EFCAB4B) Antibody (#ACC-324) can be used in western blot analysis. It has been designed to recognize CRAC channel regulator 2A from rat and human samples.

For research purposes only, not for human use
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