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Anti-D3 Dopamine Receptor (extracellular) Antibody

DRD3

Cat #: ADR-003
Alternative Name DRD3
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CGAENSTGVNRARPH, corresponding to amino acid residues 15-29 of rat DRD3 (Accession P19020). Extracellular, N-terminus.
    Accession (Uniprot) Number P19020
    Gene ID 29238
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 12/15 amino acid residues identical.
    RRID AB_2039830.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, wb
    May also work in: ic*, ip*, lci*
    Western blot
    • Western blot analysis of whole rat brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes:
      Western blot analysis of whole rat brain (lanes 1 and 3) and striatum (lanes 2 and 4) membranes:
      1,2. Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200).
      3,4. Anti-D3 Dopamine Receptor (extracellular) Antibody, preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).
    • Western blot analysis of mouse brain membranes:
      Western blot analysis of mouse brain membranes:
      1. Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:200).
      2. Anti-D3 Dopamine Receptor (extracellular) Antibody, preincubated with D3 Dopamine Receptor (extracellular) Blocking Peptide (#BLP-DR003).
    Immunohistochemistry
    • Expression of DRD3 in rat striatum 
      Expression of DRD3 in rat striatum 
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:100). A. DRD3 appears in a subset of striatal neurons (green). B. The same section was stained for parvalbumin (red), a marker of interneurons. C. Merging the two images demonstrates that DRD3 localization includes parvalbuminergic striatal interneurons. DAPI was used as the counterstain (blue).
    Indirect flow cytometry
    • Cell surface detection of D3 Dopamine Receptor by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
      Cell surface detection of D3 Dopamine Receptor by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (5μg) + goat-anti-rabbit-FITC.
    References
    1. Missale, C. et al. (1998) Physiol. Rev. 78, 189.
    2. Holmes, A. et al. (2004) Neuropharmacol. 47, 1117.
    Scientific background

    The D3 Dopamine Receptor (D3 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson’s disease and schizophrenia.1

    All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

    Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1

    The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1

    The D3 receptor distribution in the brain is relatively restricted to limbic areas such as striatum, islands of Calleja and olfactory tubercle.  In the periphery, it is expressed in the kidney particularly in proximal tubules.1

    The exact physiological function of the D3 receptor remains to be fully elucidated. In studies using D3 receptor knockout mice, the most prominent dysfunction was the development of rennin-dependent hypertension. Potential roles in reinforcement and reward behaviors have also been suggested as well as roles in neuropsychiatric disorders such as drug abuse and schizophrenia.2

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-D3 Dopamine Receptor (extracellular) Antibody
    Expression of D3 dopamine receptor in mouse L-DOPA-treated brain.
    Immunohistochemical staining of free floating mouse coronal brain sections using Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003), (1:100). DRD3 staining (green) is detected only following chronic L-DOPA treatment.Adapted from Solis, O. et al. (2017) Cereb. Cortex 27, 435. with permission of Oxford University Press.
    Last update: 08/02/2021

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus of the rat D3 dopamine receptor. Anti-D3 Dopamine Receptor (extracellular) Antibody (#ADR-003) can be used in western blot analysis and immunohistochemical applications, and recognizes DRD3 from rat and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot following immunoprecipitation of mouse striatum. Tested in DRD3 knockout mice.
      Castro-Hernandez, J. et al. (2015) Neurobiol. Dis. 74, 325
    2. Flow cytometry of CD4+ mouse T-cells. Tested in DRD3 knockout mice
      Elgueta, D. et al. (2019) Front. Immunol. 10, 981.
    Western blot citations
    1. Rat colon lysate.
      Li, Y. et al. (2019) Am. J. Physiol. 316, C393.
    2. Mouse MC3T3-E1 pre-osteoblast cell lysate.
      Lee, D.J. et al. (2015) Bone Res. 3, 15020.
    Immunohistochemistry citations
    1. Rat colon lysate.
      Li, Y. et al. (2019) Am. J. Physiol. 316, C393.
    2. Mouse brain sections.
      Montoya, A. et al. (2019) J. Neuroinflammation 16, 258.
    3. Mouse brain sections (1:100).
      Solis, O. et al. (2017) Cereb. Cortex 27, 435.
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