Anti-DLGAP1/GKAP Antibody

DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1
    Cat #: APZ-041
    Alternative Name DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)KISNEHSPKLQIRSH, corresponding to amino acid residues 397-411 of rat DLGAP1 (Accession P97836). Intracellular.
    • Anti-DLGAP1/GKAP Antibody
    Accession (Uniprot) Number P97836
    Gene ID 65040
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    RRID AB_2756784.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ip*
    Western blot
    • Anti-DLGAP1/GKAP Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
      1,2. Anti-DLGAP1/GKAP Antibody (#APZ-041), (1:200).
      3,4. Anti-DLGAP1/GKAP Antibody, preincubated with the negative control antigen.
    • Anti-DLGAP1/GKAP Antibody
      Expression of GKAP in rat hippocampus
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DLGAP1/GKAP Antibody (#APZ-041), (1:200), followed by goat anti-rabbit-AlexaFluor-488. GKAP staining (green) in hippocampal CA3 region is detected in neurons (arrows) of CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
    1. Rasmussen, A.H. et al. (2017) Mol. Brain 10, 43.
    2. Li, L. et al. (2018) Cancer Cell 33, 736.
    Scientific background

    DLGAP1/GKAP (discs large homolog associated protein 1/guanylate kinase associated protein) is a member of the DLGAP family, scaffold proteins localized to the postsynaptic density. The protein is involved in signaling to and from glutamate receptors, it serves as a key adaptor protein of NMDA receptors, involved in learning and memory and synaptic plasticity in the central nervous system1,2.

    DLGAP family comprises five conserved proteins, DLGAP1–DLGAP5. DLGAP1-DLGAP4 proteins interact directly with both DLG and SHANK through their multiple domains. DLGAP1–4 proteins are implicated in several neurological disorders including schizophrenia, autism, obsessive compulsive disorder and cerebellar ataxia1.

    DLGAP1 is highly expressed in the brain; Dlgap1 mRNA is detected in the cortex, hippocampus, olfactory bulb, striatum and thalamus1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-DLGAP1/GKAP Antibody (#APZ-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DLGAP1 from human, mouse, and rat samples.

    For research purposes only, not for human use
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