Anti-Endothelin Receptor B Antibody

EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type
    Cat #: AER-002
    Alternative Name EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide CEMLRKKSGMQIALND, corresponding to amino acid residues 298-314 of rat ET-B (Accession P21451). 3rd intracellular loop.
        Anti-Endothelin Receptor B Antibody
    Accession (Uniprot) Number P21451
    Gene ID 50672
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology All known vertebrate sequences - identical.
    RRID AB_2039840.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, wb
    May also work in: ip
      • Anti-Endothelin Receptor B Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-Endothelin Receptor B Antibody (#AER-002), (1:200).
        2. Anti-Endothelin Receptor B Antibody, preincubated with the control peptide antigen.
      • Anti-Endothelin Receptor B Antibody
        Expression of EDNRB in rat lung
        Immunohistochemical staining of paraffin embedded section of rat lung using Anti-Endothelin Receptor B Antibody (#AER-002) (1:100). EDNRB is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Note that vascular smooth muscle is not stained (blue arrows). Hematoxilin is used as the counterstain.
        Anti-Endothelin Receptor B Antibody
        Expression of EDNRB in rat thalamus
        Immunohistochemical staining of rat dorsal peri-ventricular thalamus using Anti-Endothelin Receptor B Antibody (#AER-002). Cresyl violet is used as the counterstain.
        Mouse retina (1:100) (Rattner, A. and Nathans, J. (2005) J. Neurosci. 25, 4540.).
      • Human endocardial endothelial cells (EECs) (1:100) (Jacques, D. et al. (2005) Peptides 26, 1427.).
      • Human umbilical vein endothelial cells (1:200) (Yaiw, K.C. et al. (2015) Open Forum Infect. Dis. 2, ofv155.).
    References
      • Endothelins (ET-1, 2, and 3) are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-protein coupled receptors, ETAR and ETBR. Both ETAR and ETBR are present in the heart and in human myocardium at similar levels.1,2

        The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 > ET-2 > ET-3), ETBR shows no selective affinity.2,3 

        Subsequent studies have demonstrated the presence of endothelins in vascular, as well as in non-vascular, cells and tissues, having multiple biological activities.

        Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3

        Until recently, it was thought that all cellular activities of the endothelins were mediated through their interactions with their cell surface receptors. However, a recent study demonstrated that cardiac nuclei also possess both ETAR and ETBR subtypes, which are functional and coupled to signaling mechanisms within the nuclear membrane.2

        Hypermethylation of the ETBR correlated with transcriptional down-regulation and reduced expression of ETB receptor was observed in several prostate, bladder and colon cancer cell lines.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Endothelin Receptor B Antibody
    Expression of Endothelin Receptor B in mouse JG cells.Immunocytochemical staining of mouse isolated juxtaglomerular (JG) cells using Anti-Endothelin Receptor B Antibody (#AER-002), (red). ET-B staining co-localizes with renin, a kidney cell marker, (green).Adapted from Ortiz-Capisano, M.C. (2014) Physiol. Rep. 2, e12240. with permission of the American Physiological Society and The Physiological Society.

    Last update: 31/07/2019

    Anti-Endothelin Receptor B Antibody (#AER-002) is a highly specific antibody directed against an epitope of rat ET-B. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize ET-B from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
      • Rat arterial tissue lysate.
        Amor, S. et al. (2017) Exp. Gerontol. 88, 32.
      • Rat heart lysate (1:200).
        Skovsted, G.F. et al. (2017) PLoS ONE 12, e0174119.
      • Rat spinal cord lysate.
        Forner, S. et al. (2016) Neurosci. Lett. 617, 14.
      • Human bVSMC cell lysate (1:1000).
        Yasir, A. et al. (2016) Life Sci. 159, 76.
      • Rat thick ascending limb suspension lysate (1:1250).
        Ramseyer, V.D. et al. (2015) Am. J. Physiol. 308, F149.
      • Rat coronary artery lysate.
        Skovsted, G.F. et al. (2015) Basic Clin. Pharmacol. Toxicol. 117, 297.
      • Swine femoral artery smooth muscle lysate (1:200).
        Bender, S.B. et al. (2014) J. Physiol. 592, 1757.
      • Rat kidney lysate.
        Ogura, Y. et al. (2014) Life Sci. 118, 347.
      • Rat heart lysate.
        Seki, Y. et al. (2014) Life Sci. 118, 357.
      • Human bVSMC cells.
        Yasir, A. et al. (2016) Life Sci. 159, 76.
      • Human umbilical vein endothelial cells (HUVECs), human PASMCs, human SMCs (1:500).
        Yaiw, K.C. et al. (2015) Open Forum Infect. Dis. 2, ofv155.
      • Mouse isolated juxtaglomerular isolated cells.
        Ortiz-Capisano, M.C. (2014) Physiol. Rep. 2, e12240.
      • Human endocardial endothelial cells (EECs) (1:100).
        Jacques, D. et al. (2005) Peptides 26, 1427.
      • Human umbilical vein endothelial cells (1:200).
        Yaiw, K.C. et al. (2015) Open Forum Infect. Dis. 2, ofv155.
    Related Products
        • Guinea pig Anti-Endothelin Receptor B Antibody (#AGP-037) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #AER-002.