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Anti-Ephrin-B1 (extracellular) Antibody

EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1

Cat #: AER-041
Alternative Name EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m
May also work in: r*
Immunogen
  • Peptide (C)KHETVNQEEKSGPG, corresponding to amino acid residues 208-221 of human Ephrin-B1 (Accession P98172). Extracellular domain.
Accession (Uniprot) Number P98172
Gene ID 1947
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; rat - 13/14 amino acid residues identical.
RRID AB_2756587.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse enriched brain membranes (lanes 1 and 3) and human HT-29 colon adenocarcinoma cells lysate (lanes 2 and 4):
    Western blot analysis of mouse enriched brain membranes (lanes 1 and 3) and human HT-29 colon adenocarcinoma cells lysate (lanes 2 and 4):
    1,2. Anti-Ephrin-B1 (extracellular) Antibody (#AER-041), (1:200).
    3,4. Anti-Ephrin-B1 (extracellular) Antibody, preincubated with Ephrin-B1 (extracellular) Blocking Peptide (#BLP-ER041).
References
  1. Kataoka, H. et al. (2002) J. Cancer Res. Clin. Oncol. 128, 343.
  2. Tanaka, M. et al. (2007) J. Cell Sci. 120, 2179.
  3. Pasquale, E.B. (2008) Cell 133, 38.
  4. Flanagan, J.G. et al. (1998) Ann. Rev. Neurosci. 21, 309.
Scientific background

The erythropoietin-producing hepatocellular (EPH) receptors represent the largest known family of receptor tyrosine kinases and are activated by interactions with cell-surface ligands, termed EFNs. EPH receptors have been classified into two subfamilies, EPHA and EPHB, according to their preference for either glycosylphosphatidylinositol (GPI)-anchored EFN-A ligands or transmembrane EFN-B ligands. Thus far, 14 vertebrate receptors and eight ligands have been identified1. All the ligands share a conserved core sequence of approximately 125 amino acids, including 4 invariant cysteine residues, probably corresponding to a receptor binding domain. This is followed by a membrane anchorage domain, taking the form of a GPI anchor for five of the ligands (ephrin-A1 to -A5) or a transmembrane domain for three of them (ephrin-B1 to -B3) 2.

Ephrin-B1 is signals forward or reverse signaling mechanisms. Ephrin-B1 PDZ-dependent reverse signaling controls axon guidance of the corpus callosum, whereas forward signaling is critical for normal craniofacial development2.

Eph receptors and ephrins are highly expressed in the brain and in the developing nervous system, where they are involved in fundamental developmental processes of the nervous system, including axon guidance, axonfasciculation, neural crest cell migration, acquisition of brain subregional identity,and neuronal cell survival. Ephrins are dramatically expressed in a wide range of regions of the vertebrate embryo including the ectoderm, mesoderm, and endoderm3.

Overexpression of B-type ephrin in cancer cells is reported to correlate with high invasion and high vascularity of tumors and elevated expression of ephrin-B1 is observed in poorly differentiated invasive tumor cells and other tumors with poor clinical prognosis4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Ephrin-B1. Anti-Ephrin-B1 (extracellular) Antibody (#AER-041) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the ligand in living cells. It has been designed to recognize Ephrin-B1 from human, rat and mouse samples.

For research purposes only, not for human use
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