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Anti-GPER (GPR30) Antibody

G-protein coupled estrogen receptor 1, GPER1, GPR41, Chemoattractant receptor-like 2, Cmkrl2

Cat #: AER-050
Alternative Name G-protein coupled estrogen receptor 1, GPER1, GPR41, Chemoattractant receptor-like 2, Cmkrl2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)ETFRDKLRLYVAQK, corresponding to amino acid residues 329-342 of rat GPER (Accession O08878). Intracellular, C-terminus.
Accession (Uniprot) Number O08878
Gene ID 171104
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 13/14; human - 12/14 amino acid residues identical.
RRID AB_2340934.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes:
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes:
    1-3. Anti-GPER (GPR30) Antibody (#AER-050), (1:200).
    4-6. Anti-GPER (GPR30) Antibody, preincubated with GPER/GPR30 Blocking Peptide (#BLP-ER050).
Immunohistochemistry
  • Expression of GPER in rat dentate gyrus
    Expression of GPER in rat dentate gyrus
    Immunohistochemical staining of rat hippocampal dentate gyrus using Anti-GPER (GPR30) Antibody (#AER-050). A. GPER staining (green) appears in cells and fibers of the hilus (arrows) and granule layer (G) B. Parvalbumin (red) is detected in cells in the DG hilus (arrows). C. Nuclei staining using DAPI as the counterstain (blue). D. Merge image of panels A and B reveals colocalization in some cells (arrows).
References
  1. Samartzis, E.P. et al. (2014) PLoS ONE 9, e83296.
  2. Mizukami, Y. et al. (2010) Endocr. J. 57,101.
  3. Kolkova, Z. et al. (2010) Mol. Hum. Reprod. 16, 743.
  4. Kimura, M. et al. (2001) J. Biol. Chem. 276, 26453.
  5. De Francesco, E.M. et al. (2014) Cancer Res. 74, 4053.
Scientific background

The G protein-coupled estrogen receptor (GPER), formerly known as G protein receptor 30 (GPR30), was identified as a novel estrogen receptor that mediates a rapid, non-genomic response to estrogens1.

GPER is a G protein-coupled seven-transmembrane receptor. It is believed that the N-terminus is located outside of the cell, and that aspartic acid residues in the terminal region might be modified by glycosylation if GPER is localized in the plasma membrane. It is speculated that the ligand associates with the N-terminal domain to activate the receptor. Trimeric G protein is presumed to bind to the 3rd loop of the intracellular domain based on the molecular structure. A PDZ domain appears to be in the C-terminal region of GPER, but its physiological role is unknown2.

GPER is widely distributed in the human body including: brain, liver, heart, lung, pancreas, placenta, blood vessels, bone, lymphoid tissue, as well as endometrial, ovarian and breast cancer. The receptor has been localized both in the plasma membrane and in the endoplasmic reticulum3.

It was found that GPER is rapidly induced in the cardiomyocyte ischemia model, and that its induction is associated with apoptotic cell death during ischemia and reperfusion4. In addition, the GPER mediates estrogenic signaling in breast cancer cells and cancer-associated fibroblasts (CAF) that contribute to cancer progression5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-GPER (GPR30) Antibody (#AER-050) is a highly specific antibody directed against an epitope of rat G-protein coupled estrogen receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPER from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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