Anti-GABA Transporter 2 (GAT-2) Antibody

SLC6A13, GABT2, Na+/Cl-- dependent GABA transporter 2
    Cat #: AGT-002
    Alternative Name SLC6A13, GABT2, Na+/Cl-- dependent GABA transporter 2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)KVEEDGTLEREQWTNK, corresponding to amino acids residues 23-38 of rat GAT-2 (Accession P31646). Intracellular, N-terminus.
    • Anti-GABA Transporter 2 (GAT-2) Antibody
    Accession (Uniprot) Number P31646
    Gene ID 171163
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 14/16 amino acid residues identical; human - 11/16 amino acid residues identical.
    RRID AB_10915893.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-GABA Transporter 2 (GAT-2) Antibody
      Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
      1,2. Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002), (1:200).
      3,4. Anti-GABA Transporter 2 (GAT-2) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-GABA Transporter 2 (GAT-2) Antibody
      Expression of GABA transporter 2 in rat lung
      Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002), (1:100). GAT-2 (brown) is expressed in the respiratory epithelium of the bronchioli. Hematoxilin is used as the counterstain.
    References
    1. Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175.
    2. Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375.
    3. Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226.
    4. Christiansen, B. et al. (2007) J. Biol. Chem. 282, 19331.
    Scientific background

    γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.

    To date, four GATs have been identified: GAT-1, GAT-2GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3.

    GAT-2 is expressed in the brain and in the periphery and is also abundant in the kidney and liver4. Due to its relatively broad expression pattern, suggested roles for GAT-2 include maturation of the nervous system, and regulation of GABA levels among others4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat GABA transporter 2. Anti-GABA Transporter 2 (GAT-2) Antibody (#AGT-002) can be used in western blot analysis and immunohistochemistry applications and has been designed to recognize GAT-2 from rat, mouse and human samples.

    For research purposes only, not for human use
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