Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody

γ-Aminobutyric acid receptor type A subunit α1, GABRA1
    Cat #: AGP-083
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: h, m, r
    Immunogen
    Peptide QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1 (Accession P62813). Extracellular, N-terminus.
    Accession (Uniprot) Number P62813
    Gene ID 29705
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 16/17 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
    Western blot
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) membranes and human brain neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell lysate:
    1-3. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083), (1:400).
    4-6. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Following a broad screen of secondary antibodies, the following were used for these applications:
    Western blot analysis: #106-035-006 (Jackson ImmunoResearch) or #A7289 (SIGMA).
    Immunohistochemistry
    Expression of GABA(A) α1 Receptor in rat hippocampus
    Immunohistochemical staining of rat hippocampal dentate gyrus sections using Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083). A. GABRA1 staining (red) appears in neuronal soma (horizontal arrows in panel B) and in the neuronal processes (vertical arrows in panel B). B. GABRA1 staining merged with DAPI counterstain (blue).
    References
    1. Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci3, 71.
    2. Whiting, P.J. (1999) Neurochem. Int. 34, 387.
    3. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127.
    4. Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057.
    5. Fuchs, K. and Celepirovic, N. (2002) J. Neurochem82, 1512.
    Scientific background

    GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.1

    The GABA transmitter interacts with two major types of receptors: ionotropic GABAAreceptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.2 GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl-channel, producing an increase in membrane conductance that results in inhibition of neural activity.2

    GABAARs are heteropentamers, in which all five subunits contribute to pore formation.  To date, eight subunit isoforms have been cloned:α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consists of 2α, 2β, and 1δ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands.

    Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes.4 The α1 subunit is highly expressed in adulthood while the α2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the α-subunits that are highly expressed in early development (α2, α3, and α5) and those expressed in adulthood (α1) is suggested to play a major role in the development of temporal lobe epilepsy.5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Guinea pig Anti-GABA(A) α1 Receptor (extracellular) AntibodyImmuno-colocalization of Synapsin-2 and GABA(A) α1 Receptor in rat striatum.Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-Synapsin-2 Antibody (#ANR-015), (1:300) and Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083), (1:600). A. Synapsin-2 staining (red) appears in Islands of Calleja (IC), (arrow). B. GABRA1 (green) is also detected in Islands of Calleja and in adjacent neurons. C. Merge of the two images demonstrates colocalization in IC while adjacent GABRA1 positive neurons do not express Synapsin-2. Cell nuclei are stained with DAPI (blue).

    Last update: 31/10/2018

    Alomone Labs offers a highly specific antibody directed against an extracellular epitope of rat GABA (A) α1 receptor. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083) raised in guinea pig can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is highly suited to detect GABRA1 in live cells. It has been designed to recognize GABRA1 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use