Anti-GABA(A) α2 Receptor Antibody

γ-Aminobutyric acid receptor type A subunit α2, GABRA2
    Cat #: AGA-002
    Alternative Name γ-Aminobutyric acid receptor type A subunit α2, GABRA2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)TPEPNKKPENKPA, corresponding to amino acid residues 393-405 of rat GABRA2 (Accession P23576). 2nd intracellular loop.
        Anti-GABA(A) α2 Receptor Antibody
    Accession (Uniprot) Number P23576
    Gene ID 289606
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human, bovine - identical.
    RRID AB_2039864.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-GABA(A) α2 Receptor Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-GABA(A) α2 Receptor Antibody (#AGA-002), (1:200).   
        2. Anti-GABA(A) α2 Receptor Antibody, preincubated with the negative control antigen.
      • Anti-GABA(A) α2 Receptor Antibody
        Expression of GABRA2 in mouse cerebellum
        Immunohistochemical staining of mouse formalin-fixed frozen cerebellum sections with Anti-GABA(A) α2 Receptor Antibody (#AGA-002). The receptor subunit is localized mainly on the soma of Purkinje cells. Staining with mouse anti-parvalbumin (red) suggests that GABRA2 subunit is restricted to a sub-region of the Purkinje cell.
      • Mouse adult hippocampal neural stem cells (1:200) (Quadrato, G. et al. (2014) J. Neurosci. 34, 8630.).
    References
      • GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.1 

        The GABA transmitter interacts with two major types of receptors: ionotropic GABAA (GABAAR) and metabotropic GABAB receptors. The GABAAR belong to the ligand gated ion channel superfamily.2 

        The GABAAR, is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms were described to exist in mammals (α1-α6). The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ submit. The α subunit is the most common subunit and is expressed ubiquitously. It determines the affinities for allosteric ligands shown by GABAAR. 

        α2-Subunit mRNA is strongly expressed only in the hippocampus and the hypothalamus. It occurs very early in rat brain development and declines in adulthood. 

        The failure to complete the normal transition between the α-subunits that are highly expressed in early development (α2, α3, α5) to those expressed in adulthood (α1) might play a major role in the development of temporal lobe epilepsy.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Alomone Labs offers a highly specific antibody directed against an epitope of the rat GABA (A) α2 receptor. Anti-GABA(A) α2 Receptor Antibody (#AGA-002) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize GABRA2 from human, rat and mouse samples.

    For research purposes only, not for human use
    Citations
      • Mouse RAW264.7 macrophage cell lysate.
        Januzi, L. et al. (2018) Cell. Immunol. 332, 7.
      • Mouse lung sections (1:200).
        Januzi, L. et al. (2018) Cell. Immunol. 332, 7.
      • Rat DRG sections.
        Kannampalli, P. et al. (2017) Neuroscience 346, 349.
      • Mouse brain sections (1:250).
        Skilbeck, K.J. et al. (2018) Pharmacol. Res. 128, 179.
      • Mouse adult hippocampal neural stem cells (1:200).
        Quadrato, G. et al. (2014) J. Neurosci. 34, 8630.
      • Yue, J. et al. (2018) Brain Res. Bull. 137, 156.
      • Hanson, J.E. et al. (2013) J. Neurosci. 33, 5924.
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