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Anti-GABA(B) R1 (extracellular) Antibody

γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A
Cat #: AGB-001
Alternative Name γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
  • Peptide CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABBR1 (Accession Q9Z0U4). Extracellular, N-terminus.
Accession (Uniprot) Number Q9Z0U4
Gene ID 81657
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human, canine, bovine - identical.
RRID AB_2039857.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Does not recognize alternative splice 1b.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:200).
    2. Anti-GABA(B) R1 (extracellular) Antibody, preincubated with GABA(B) R1 (extracellular) Blocking Peptide (#BLP-GB001).
  • Expression of GABA(B) receptor 1 in mouse hippocampus
    Expression of GABA(B) receptor 1 in mouse hippocampus
    Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:100). A. GABBR1 staining (green) is detected in neurons in the CA3 field and in the dentate granule layer (short arrows), as well as in dendrites of CA3 pyramidal neurons (long arrows). B. Staining with mouse anti-GAP43 antibody (red) sets apart the stratum lacunosum moleculare (SLM). C. Confocal merge suggests the presence of GABBR1 in pyramidal neurons.
  1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109.
  2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835.
  3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035.
  4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043.
  5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14649.
  6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14643.
Scientific background

GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2

The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA (B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4

The functional GABA (B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 The GABA(B) R1 subunit was demonstrated to be important for agonist and antagonist binding, while GABA(B) R2 was shown to be essential for trafficking and for G-protein binding.

To date, eight alternatively spliced isoforms of GABA (B) R1 have been proposed. These are named 1a-1h, of which 1a and 1b are the most prominent. Only 1a, 1b, and 1c appear to act as functional subunits.6,7 They are widespread, being expressed in the testis, stomach, spinal cord, and brain. Isoform 1b is also expressed in the kidney and liver.8

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat GABA(B) receptor 1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR1 from human, rat, and mouse samples.

For research purposes only, not for human use



Scientific Background


Immunohistochemistry citations
  1. Rat DRG sections.
    Liu, Z. et al. (2016) Mol. Pain 12, 1.
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