Anti-GABA(B) R2 Antibody

γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20
    Cat #: AGB-002
    Alternative Name γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABBR2 (Accession O75899). Intracellular, C-terminus.
        Anti-GABA(B) R2 Antibody
    Accession (Uniprot) Number O75899
    Gene ID 9568
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, bovine - identical.
    RRID AB_2039859.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-GABA(B) R2 Antibody
        Western blot analysis of rat brain membrane:
        1. Anti-GABA(B) R2 Antibody (#AGB-002), (1:200).
        2. Anti-GABA(B) R2 Antibody, preincubated with the negative control antigen.
      • Anti-GABA(B) R2 Antibody
        Expression of GABA(B) receptor 2 in mouse hippocampus
        Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 Antibody (#AGB-002 ), (1:100). A. GABBR2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABBR2 in layers, but not in pyramidal neurons.
    References
      • GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2

        The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA(B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4

        The functional GABA(B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6

        GABA(B) R2 has been shown to be essential for trafficking and for G-protein binding while the GABA(B) R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA(B) R2 is highly expressed in the brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in the testis.7

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human GABA(B) receptor 2. Anti-GABA(B) R2 Antibody (#AGB-002), can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR2 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • HEK-293 cells stably expressing human GABA(B) R2.
        Han, C. et al. (2013) J. Med. Chem. 56, 2465.
      • Rat DRG sections.
        Liu, Z. et al. (2016) Mol. Pain 12, 1.
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