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Anti-GABA(B) R2 Antibody

γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20
Cat #: AGB-002
Alternative Name γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABBR2 (Accession O75899). Intracellular, C-terminus.
Accession (Uniprot) Number O75899
Gene ID 9568
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, bovine - identical.
RRID AB_2039859.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain membrane:
    Western blot analysis of rat brain membrane:
    1. Anti-GABA(B) R2 Antibody (#AGB-002), (1:200).
    2. Anti-GABA(B) R2 Antibody, preincubated with GABA(B) R2 Blocking Peptide (#BLP-GB002).
Immunohistochemistry
  • Expression of GABA(B) receptor 2 in mouse hippocampus
    Expression of GABA(B) receptor 2 in mouse hippocampus
    Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 Antibody (#AGB-002 ), (1:100). A. GABBR2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABBR2 in layers, but not in pyramidal neurons.
References
  1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109.
  2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835.
  3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035.
  4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043.
  5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14649.
  6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14643.
  7. http://www.uniprot.org/uniprot/O75899.
Scientific background

GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2

The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA(B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4

The functional GABA(B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6

GABA(B) R2 has been shown to be essential for trafficking and for G-protein binding while the GABA(B) R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA(B) R2 is highly expressed in the brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in the testis.7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human GABA(B) receptor 2. Anti-GABA(B) R2 Antibody (#AGB-002), can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR2 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. HEK-293 cells stably expressing human GABA(B) R2.
    Han, C. et al. (2013) J. Med. Chem. 56, 2465.
Immunohistochemistry citations
  1. Rat DRG sections.
    Liu, Z. et al. (2016) Mol. Pain 12, 1.
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