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Anti-GLP2R (extracellular) Antibody

Glucagon-like peptide 2 receptor, GLP-2 receptor

Cat #: AGR-022
Alternative Name Glucagon-like peptide 2 receptor, GLP-2 receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
May also work in: h*, m*
Immunogen
  • Peptide (C)KRPDDESGWMSYLSE, corresponding to amino acid residues 241-255 of rat GLP2R (Accession Q9Z0W0). 1st extracellular loop.
Accession (Uniprot) Number Q9Z0W0
Gene ID 60432
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - 13/15 amino acid residues identical.
RRID AB_10917758.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat colon lysate:
    Western blot analysis of rat colon lysate:
    1. Anti-GLP2R (extracellular) Antibody (#AGR-022), (1:200).
    2. Anti-GLP2R (extracellular) Antibody, preincubated with GLP2R (extracellular) Blocking Peptide (#BLP-GR022).
Immunohistochemistry
  • Expression of Glucagon-like peptide 2 receptor in rat hypothalamus
    Expression of Glucagon-like peptide 2 receptor in rat hypothalamus
    Immunohistochemical staining of rat hypothalamus with Anti-GLP2R (extracellular) Antibody (#AGR-022), (1:200). Glucagon-like peptide 2 receptor (green) appears in the lateral hypothalamic region axonal profiles (vertical white arrow) and nerve cell profiles (horizontal white arrows) were labeled. DAPI is used as the counterstain (blue).
References
  1. Dubé, P.E. and Brubaker, P.L. (2007) Am. J. Physiol. Endocrinol. Metab. 293, E460.
  2. Wallis, K. et al. (2007) Aliment. Pharmacol. Ther. 25, 365.
  3. Mayo, K.E. et al. (2003) Pharmacol. Rev. 55, 167.
Scientific background

Glucagon like peptide-2 (GLP-2) is a 33 amino acid peptide secreted following food consumption. It is cleaved by dipeptidyl peptidase IV thereby yielding a very short half life. GLP-2 imposes most of its effect on intestinal epithelial cells where it is also be used as a therapeutic drug for gastrointestinal injuries1-3.
The many actions of GLP-2 include cellular proliferation of intestinal epithelial cells, the increase of amino acid and lipid absorption and increase of digestive enzyme activity of the epithelial brush border cells1.

The GLP-2 cellular and physiological effects are primarily exerted through the glucagon-like peptide-2 receptor (GLP-2R), a member of the G-protein coupled receptor superfamily. The receptor can couple either Gs or Gi G-proteins1. However, when heterologously expressed, the receptor can stimulate the synthesis of cAMP3.

Expression of GLP-2R is strongly detected in the central nervous system and the gastrointestinal tract, although its expression in this area remains controversial as some species express the receptor in different regions than others. It is also slightly detected in the lungs, cervix and vagal afferents1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-GLP2R (extracellular) Antibody (#AGR-022) is a highly specific antibody directed against an epitpe of the rat glucagon-like peptide 2 receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLP2R from human, rat, and mouse samples.

For research purposes only, not for human use
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