Free shipping starts now, no minimum, no coupons required!

Anti-GLUT4 Antibody

Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4

Cat #: AGT-024
Alternative Name Glucose transporter 4, Facilitated glucose transporter member 4, Insulin-responsive glucose transporter type 4, SLC2A4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KPSTELEYLGPDE, corresponding to amino acid residues 495-507 of human Glucose Transporter 4 (Accession P14672). Intracellular, C-terminus.
Accession (Uniprot) Number P14672
Gene ID 6517
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse – identical.
RRID AB_2631197.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes:
    1,2. Anti-GLUT4 Antibody (#AGT-024), (1:400).
    3,4. Anti-GLUT4 Antibody, preincubated with GLUT4 Blocking Peptide (#BLP-GT024).
Immunohistochemistry
  • Expression of Glucose Transporter 4 in rat olfactory bulb
    Expression of Glucose Transporter 4 in rat olfactory bulb
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GLUT4 Antibody (#AGT-024), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. GLUT4 staining (green) appears in some glomeruli (“G” and horizontal arrows), but not in others (vertical arrow). Cell nuclei are stained with DAPI (blue).
References
  1. Lacombe, V.A. (2014) ISRN Vet Sci. 2014, 409547.
  2. Stöckli, J. et al. (2011) J. Cell Sci. 124, 41472.
Scientific background

The GLUT protein family is a member of the major facilitator superfamily of membrane transporters and is encoded by the SLC2 genes. GLUT proteins contain 12 membrane-spanning domains, an N-linked glycosylation site, and intracellular NH2 and COOH termini. In addition there are several conserved residues and motifs designated “sugar transporter signatures”.

To date, 14 GLUT isoforms have been identified and divided into 3 different classes based on sequence similarity and structural and functional characteristics. GLUT4 together with GLUT 1-3 and 14 belong to Class I.

GLUT4 is an insulin-regulated glucose transporter responsible for insulin-regulated glucose uptake into fat and muscle cells. GLUT4 translocates from intracellular stores to the cell surface, from an inactive site to an active one in order to enhance glucose uptake1.

GLUT4 is expressed mainly in adipose tissue and skeletal muscle. It is also detected in brain, kidney and intestine.

There is no evidence for mutation in GLUT4 that is linked to metabolic disease in humans. However, GLUT4 polymorphisms are rare and equally common in normoglycaemic individuals and type 2 diabetics. General decrease in GLUT4 levels show signs of diabetes, while a disruption in GLUT4 expression in muscle or adipose tissue might contribute to the development of whole-body insulin resistance in humans2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Anti-GLUT4 Antibody (#AGT-024) is a highly specific antibody directed against an epitope of the human Glucose Transporter 4 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GLUT4 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information