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Anti-GLUT1 (extracellular) Antibody

Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1
Cat #: AGT-041
Alternative Name Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EEFYNQTWNHRYGES, corresponding to amino acid residues 41-55 of rat Glucose transporter 1 (Accession P11167). 1st extracellular loop.
Accession (Uniprot) Number P11167
Gene ID 24778
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – 14 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-GLUT1 (extracellular) Antibody (#AGT-041), (1:400).
    3,4. Anti-GLUT1 (extracellular) Antibody, preincubated with GLUT1 (extracellular) Blocking Peptide (#BLP-GT041).
  • Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 5), human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 6), human K562 chronic myeloid leukemia cell line lysate (lanes 3 and 7) and human Jurkat T-cell leukemia cell line lysate (lanes 4 and 8):
    Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 5), human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 6), human K562 chronic myeloid leukemia cell line lysate (lanes 3 and 7) and human Jurkat T-cell leukemia cell line lysate (lanes 4 and 8):
    1-4. Anti-GLUT1 (extracellular) Antibody (#AGT-041), (1:400).
    5-8. Anti-GLUT1 (extracellular) Antibody, preincubated with GLUT1 (extracellular) Blocking Peptide (#BLP-GT041).
Indirect flow cytometry
  • Cell surface detection of GLUT1 in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of GLUT1 in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GLUT1 (extracellular) Antibody (#AGT-041), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Olson, A.L. et al. (1996) Annu. Rev. Nutr 16, 235.
  2. Pardridge, W.M. et al. (1990) J. Biol. Chem265, 18035.
  3. Klepper, J. et al. (1999) Neurochem. Res. 24, 587.
  4. Leen, W.G. et al. (2014) J. Neurol. 261, 589.
  5. Amann, T. et al. (2011) Mol. Membr. Biol28, 182.
Scientific background

Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS). It is encoded by SLC2A1, and mediates basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present1.

GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue2.

Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain3. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development4. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-GLUT1 (extracellular) Antibody (#AGT-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GLUT1 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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