Name: Anti-GPR142 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AGR-082-F

Overview

Cat #: AGR-082-F

Alternative Name G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142 (Accession Q7TQN9). Extracellular, 3^rd loop.

Accession (Uniprot) Number Q7TQN9

Gene ID 217302

Homology Rat, human – identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-082), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of GPR142 by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-GPR142 (extracellular)-FITC Antibody (#AGR-082-F), (5µg).
Cell surface detection of GPR142 by direct flow cytometry in live intact mouse J774 macrophage cell line:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-GPR142 (extracellular)-FITC Antibody (#AGR-082-F), (5µg).

References

Kamato, D. et al. (2015) Front. Cardiovasc. Med., 2, 14.
Kaushik, C.A. et al. (2018) J. Biomol. Struct. Dyn., 36, 1788.
Al-Amily, I.M. et al. (2019) Pflugers. Arch. – Eur. J. Physiol. 471, 633.
Murakoshi, M. et al. (2017) Journal of Receptors and Signal Transduction, 37(3), 290-296.

Scientific background

G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs¹.

The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33% amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 – 1 mM². GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management³.

Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR142 (extracellular) Antibody (#AGR-082) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR142 from rat, mouse and human samples.

Anti-GPR142 (extracellular)-FITC Antibody (#AGR-082-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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Anti-GPR142 (extracellular)-FITC Antibody