Name: Anti-GPR34 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AGR-055-F
Availability: InStock

Cat #: AGR-055-F

Alternative Name G-protein coupled receptor 34

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CFHYRDRHNAKGE, corresponding to amino acid residues 197-209 of mouse GPR34 (Accession Q9R1K6). 2^nd extracellular loop.

Accession (Uniprot) Number Q9R1K6

Gene ID 23890

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human, rat – 12/13 amino acid residues identical.

RRID AB_2876804

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-055), and direct flow cytometry (labeled antibody).

Applications: fc

May also work in: ic*, lci*

Direct flow cytometry

Mouse BV-2 microglia cells; human THP-1 monocytic leukemia cells (2.5 µg).
Cell surface detection of GPR34 in live intact mouse BV-2 microglia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-GPR34 (extracellular)-FITC Antibody (#AGR-055-F), (2.5 µg).

Scientific background

The G-protein-coupled receptor 34 (GPR34) is currently classified as an orphan receptor of the P2Y₁₂-like receptor group. The receptor belongs to the super family of G-protein coupled receptors (GPCRs)¹.

The N-terminus of human GPR34 has three putative N-glycosylation sites (NXS/T), and the C-terminus has a cysteine residue which is palmitoylated in many GPCRs. GPR34 contains several putative phosphorylation sites primed by a serine or threonine in the C-terminus².

GPR34 is highly expressed in immune cells such as mast cells, macrophages, microglia, and immune system–derived cell lines (HL-60, K562, WEHI-3B, RAW 264.7, P815). Studies show that GPR34 function is required to arrest microglia in the M0 homeostatic non-phagocytic phenotype and downregulation of GPR34 promotes the neurodegenerative microglia phenotype^2,3,4.

GPR34 is linked to several human diseases including immunological diseases and cancer. Most known effects of GPR34 are immune function-related. For example, GPR34 is significantly down-regulated in Mycobacterium tuberculosis-infected macrophages; GPR34 mRNA is significantly lower in the cerebrospinal fluid from multiple sclerosis patients; GPR34 is significantly upregulated in the striatum and cortex of patients with Parkinson's disease but downregulated in substantia nigra. elevated GPR34 also plays a role in the progression of some tumors, such as human gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and colorectal cancer^1-3.

Lyophilized Powder

Last update: 11/04/2021

For research purposes only, not for human use

Specifications

Applications

Scientific Background

Citations

Shipping and Ordering information

Order Summary

Certificate of Analysis

Anti-GPR34 (extracellular)-FITC Antibody

Application key:

Species reactivity key:

Specifications

Applications

Scientific Background

Citations

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